EVALUATION OF CISPLATIN INDUCED GENOTOXICITY IN MALE SPRAGUE DAWLEY RATS

Abstract

Objective: To study cisplatin induced genotoxicity in reticulocytes and colonic epithelial cells of male Sprague Dawley rats. Study Design: Randomized controlled trial. Place and Duration of Study:  Study was conducted at Department of Physiology, Army Medical College, Rawalpindi from Oct 2014 to Apr 2016. Methodology: Trial was conducted on sixty male Sprague Dawley rats having average age 80-90 days and weight 250±50 grams. Rats were randomly allocated into two groups with 30 rats each. Rats in group I were administered intraperitoneal normal saline 10 ml/kg body weight twice a week for 4 weeks whereas rats in group II received intraperitoneal cisplatin 2 mg/kg body weight twice a week for 4 weeks. After 4 weeks, animals were sacrificed and blood sample was obtained for evaluation of serum malondialdehyde and micronucleated reticulocytes. After collection of blood sample, rats were dissected and small part of colon was excised and studied for micronucleated colonic epithelial cells. Results: In group I, mean micronucleated reticulocytes were (0.09±0.07%), micronucleated colonic epithelial cells were (0.24±0.11%) and serum malondialdehyde was (3.3±0.35 µmol/L) whereas in group II, mean micronucleated reticulocytes were (0.33±0.12%), micronucleated colonic epithelial cells were (2.97±0.47%) and serum malondialdehyde was (7.9±0.46 µmol/L). Results of all three variables were significantly raised (p<0.001) in group II as compared to group I. Conclusion: Cisplatin administration produced oxidative stress by increased generation of reactive oxygen species which consequently resulted in genotoxicity in male Sprague Dawley rats.

Keywords: Cisplatin,Genotoxicity, Sprague Dawley rats, Oxidative stress, Micronucleus

Pak J Physiol 2019;15(1):21-4