SERUM MALONDIALDEHYDE AS A MARKER OF OXIDATIVE STRESS IN GESTATIONAL DIABETES MELLITUS: A COMPARATIVE STUDY
DOI:
https://doi.org/10.69656/pjp.v21i3.1836Keywords:
gestational diabetes mellitus, hyperglycemia, oxidative stress, malondialdehydeAbstract
Background: Oxidative stress is implicated in causation of many diseases including diabetes mellitus. An abnormally high level of oxidative stress may be involved in development of gestational diabetes mellitus (GDM). We aimed to compare degree of oxidative stress in healthy pregnant, and GDM women by measuring serum malondialdehyde (MDA) levels. Methods: This comparative cross-sectional study was conducted on age and gestational age matched 30 healthy pregnant women and 30 patients of GDM at Army Medical College and Pak Emirates Military Hospital, Rawalpindi over a period of one year. The diagnosis of GDM was made during second trimester after oral glucose tolerance test. The subjects with type-1 or type-2 diabetes, past history of gestational diabetes and systemic inflammatory disease were not included. Serum malondialdehyde levels of all subjects were measured with ELISA technique. Data were analysed on SPSS-22. Numerical data were expressed as Mean±SD and the comparison between two groups was done using independent samples t-test. The Pearson’s correlation coefficient for association between numerical variables was assessed, and p?0.05 was regarded statistically significant. Results: GDM group had significantly higher mean serum MDA level as compared to healthy pregnant women. Serum MDA had positive correlation with fasting plasma glucose and glycosylated haemoglobin. Conclusion: A significantly high MDA in GDM along with positive correlation with fasting glucose and glycosylated haemoglobin indicates the possible role of oxidative stress in GDM.
Pak J Physiol 2025;21(3):3–6, DOI: https://doi.org/10.69656/pjp.v21i3.1836
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Saucedo R, Ortega-Camarillo C, Ferreira-Hermosillo A, Díaz-Velázquez MF, Meixueiro-Calderón C, Valencia-Ortega J. Role of oxidative stress and inflammation in gestational diabetes mellitus. Antioxidants 2023;12(10):1812.
Saeedi M, Cao Y, Fadl H, Gustafson H, Simmons D. Increasing prevalence of gestational diabetes mellitus when implementing the IADPSG criteria: A systematic review and meta-analysis. Diabetes Res Clin Pract 2021;172:108642.
Gyasi-Antwi P, Walker L, Moody C, Okyere S, Salt K, Anang L, et al. Global prevalence of gestational diabetes mellitus: a systematic review and meta-analysis. New Am J Med 2020;1(3):1–10.
Moon JH, Jang HC. Gestational diabetes mellitus: diagnostic approaches and maternal-offspring complications. Diabetes Metab J 2022;46(1):3–14.
Reitzle L, Heidemann C, Baumert J, Kaltheuner M, Adamczewski H, Icks A, et al. Pregnancy complications in women with pregestational and gestational diabetes mellitus. Dtsch Arztebl Int 2023;120(6):81.
Phoswa WN, Khaliq OP. The role of oxidative stress in hypertensive disorders of pregnancy (preeclampsia, gestational hypertension) and metabolic disorder of pregnancy (gestational diabetes mellitus). Oxid Med Cell Longev 2021;2021(1):5581570.
Caturano A, D’Angelo M, Mormone A, Russo V, Mollica MP, Salvatore T, et al. Oxidative stress in type 2 diabetes: impacts from pathogenesis to lifestyle modifications. Curr Issues Mol Biol 2023;45(8):6651–66.
Lappas M, Hiden U, Desoye G, Froehlich J, Hauguel-de Mouzon S, Jawerbaum A. The role of oxidative stress in the pathophysiology of gestational diabetes mellitus. Antioxid Redox Signal 2011;15(12):3061–100.
Zheng ZX, Feng X, Zhuang L. The effect of oxidative stress and antioxidants treatment on gestational diabetes mellitus outcome: a scoping review. Cell Biochem Biophys 2024;82(4):3003–13.
Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford University Press, USA; 2015.
Qadir SY, Yasmin T, Fatima I. Maternal and foetal outcome in gestational diabetes. J Ayub Med Coll Abbottabad 2012;24(3-4):17–20.
Qin Z, Du Y, Wang Z, Qin X, Wu H, Yu X, et al. Serum ferritin levels in pregnancy and their association with gestational diabetes mellitus: A prospective longitudinal study. Diabetes Metab Syndr Obes 2025:413–22.
Arslan A. Serum ischemia modified albumin and oxidative stress levels in patients with gestational diabetes mellitus. East J Med 2022;27(4):507–12.
Zhang C, Yang Y, Chen R, Wei Y, Feng Y, Zheng W, et al. Aberrant expression of oxidative stress related proteins affects the pregnancy outcome of gestational diabetes mellitus patients. Am J Transl Res 2019;11(1):269–79.
Akinjiyan MO, Atiba AS, Asaolu MF. Effects of gestational diabetes mellitus and diabetes mellitus on lipid profile, antioxidants, hormones and electrolytes status in a population of Nigerian women. Cardiovascular Diabetology-Endocrinology Reports 2025;11(1):1.
Bulut A, Akca G, Aktan AK, Akbulut KG, Babül A. The significance of blood and salivary oxidative stress markers and chemerin in gestational diabetes mellitus. Taiwan J Obstet Gynecol 2021;60(4):695–9.
Sudharshana Murthy KA, Bhandiwada A, Chandan SL, Gowda SL, Sindhusree G. Evaluation of oxidative stress and proinflammatory cytokines in gestational diabetes mellitus and their correlation with pregnancy outcome. Indian J Endocrinol Metab 2018;22(1):79–84.
Ibrahim A, Khoo MI, Ismail EHE, Hussain NHN, Zin AAM, Noordin L, et al. Oxidative stress biomarkers in pregnancy: a systematic review. Reprod Biol Endocrinol 2024;22(1):93.
López-Tinoco C, Roca M, García-Valero A, Murri M, Tinahones FJ, Segundo C, et al. Oxidative stress and antioxidant status in patients with late-onset gestational diabetes mellitus. Acta Diabetol 2013;50:201–8.
Shang M, Zhao J, Yang L, Lin L. Oxidative stress and antioxidant status in women with gestational diabetes mellitus diagnosed by IADPSG criteria. Diabetes Res Clin Pract 2015;109(2):404–10.
Nishikawa T, Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, et al. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 2000;404(6779):787–90.
Davì G, Guagnano MT, Ciabattoni G, Basili S, Falco A, Marinopiccoli M, et al. Platelet activation in obese women: role of inflammation and oxidant stress. JAMA 2002;288(16):2008–14.
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Copyright (c) 2025 Tallat Naureen, Syeda Fouzia Mazloum, Laiba Shaukat, Qural UL ain Fatima, Sadia Mubarak, Mahvash Khan

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