SHIELDING EFFECTS OF CORN SILK EXTRACT AGAINST DOXORUBICIN INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS
DOI:
https://doi.org/10.69656/pjp.v21i1.1779Keywords:
Congestion, Corn silk extract, Doxorubicin, Hepatoxicity, SinusoidAbstract
Background: Doxorubicin is a known anticancer drug. This study evaluated the protective effects of corn silk extract on hepatotoxicity induced by Doxorubicin in albino rats. Methods: This randomized control trial was conducted in animal house of Anatomy Department, PGMI, Lahore, for one year in 2022. Thirty healthy adult albino rats of either sex, aging 6–8 weeks with 180–220 g of weight were randomly allotted to three groups 1 (control), 2 (experimental group-1), and 3 (experimental group-2) (n=10) that were given standard rat feed and distilled water ad libitum. Intraperitonially 1.2 mg/Kg body weight distilled water and doxorubicin were given to group 1 and group 2+3 for 21 days respectively. Group-3 was also given corn silk extract 400 mg/Kg/body weight by oral gavage for 21 days. On 22nd day, blood was drawn for biochemical analysis of alkaline phosphatase and gamma glutamyl transpeptidase, and liver were preserved for tissue processing and histological analysis. Results: Serum alkaline phosphatase of group 1 was 134.70±15.18, group 2 was 328.30±18.05, and group 3 was 229.9±17.9. The serum gamma glutamyl transpeptidase of group 1 was 2.6±1.4, group 2 was 17.63±1.4, and group 3 was 9.6±1.2 (p<0.01). Histological analysis showed that group 1 rats had normal liver histology. Sinusoidal congestion and granuloma formation was observed in all rats of group 2. While in group 3, minimal sinusoidal congestion and granuloma formation was seen. Conclusion: Corn silk extract has protective effects against doxorubicin induced liver damage.
Pak J Physiol 2025;21(1):60?3, DOI: https://doi.org/10.69656/pjp.v21i1.1779
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References
Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2021;71(3):209–49.
Liu B, Zhou H, Tan L, Siu KTH, Guan XY. Exploring treatment options in cancer: Tumor treatment strategies. Signal Transduct Target Ther 2024;9(1):175.
Kciuk M, Gieleci?ska A, Mujwar S, Ko?at D, Ka?uzi?ska-Ko?at ?, Celik I, et al. Doxorubicin—an agent with multiple mechanisms of anticancer activity. Cells 2023;12(4):659.
Sritharan S, Sivalingam N. A comprehensive review on time-tested anticancer drug doxorubicin. Life Sci 2021;278:119527.
Sheibani M, Azizi Y, Shayan M, Nezamoleslami S, Eslami F, Farjoo MH, et al. Doxorubicin-induced cardiotoxicity: an overview on pre-clinical therapeutic approaches. Cardiovasc Toxicol 2022;22(4):292–310.
Hadi HM, Shahada AR, Hussein NM, Hussein EHA. Doxorubicin side effects and its uses a new update: a narrative review. Arabian J Drug Res 2021;1(1):1-6.
Shukla SK, Sarode A, Wang X, Mitragotri S, Gupta V. Particle shape engineering for improving safety and efficacy of doxorubicin—a case study of rod-shaped carriers in resistant small cell lung cancer. Biomater Adv 2022;137:212850.
van der Zanden SY, Qiao X, Neefjes J. New insights into the activities and toxicities of the old anticancer drug doxorubicin. FEBS J 2021;288(21):6095–111.
Clayton ZS, Brunt VE, Hutton DA, VanDongen NS, D’Alessandro A, Reisz JA, et al. Doxorubicin-induced oxidative stress and endothelial dysfunction in conduit arteries is prevented by mitochondrial-specific antioxidant treatment. JACC: CardioOncol 2020;2(3):475–88.
Owumi SE, Lewu DO, Arunsi UO, Oyelere AK. Luteolin attenuates doxorubicin-induced derangements of liver and kidney by reducing oxidative and inflammatory stress to suppress apoptosis. Hum Exp Toxicol 2021;40(10):1656–72.
Oros-Pantoja R, Córdoba-Adaya JC, Torres-García E, Morales-Avila E, Aranda-Lara L, Santillán-Benítez JG, et al. Preclinical evaluation of early multi-organ toxicity induced by liposomal doxorubicin using 67Ga-citrate. Nanotoxicology 2022;16(2):247–64.
Kumar JB, Goud BM, Kumar A. Liver Function Tests: Biochemical overview for clinical correlation. Indian J Med Biochem 2021;25(1):31–7.
Umbaugh DS, Jaeschke H. Biomarkers of drug-induced liver injury: A mechanistic perspective through acetaminophen hepatotoxicity. Expert Rev Gastroenterol Hepatol 2021;15(4):363–75.
Gasmi B, Kleiner DE. Liver histology: diagnostic and prognostic features. Clin Liver Dis 2020;24(1):61–74.
Calistri L, Rastrelli V, Nardi C, Maraghelli D, Vidali S, Pietragalla M, et al. Imaging of the chemotherapy-induced hepatic damage: Yellow liver, blue liver, and pseudocirrhosis. World J Gastroenterol 2021;27(46):7866–93.
Lap?ík L, ?epka D, Lap?íková B, Sumczynski D, Gautam S, Li P, et al. A physicochemical study of the antioxidant activity of corn silk extracts. Foods 2023;12(11):2159.
Adetunji CO, Samuel MO, Adetunji JB, Oluranti OI. Corn silk and health benefits. In: Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics. Boca Raton: CRC Press; 2022. p. 167–78.
Singh J, Inbaraj BS, Kaur S, Rasane P, Nanda V. Phytochemical analysis and characterization of corn silk (Zea mays, G5417). Agronomy 2022;12(4):777.
Maneechote C, Khuanjing T, Ongnok B, Arinno A, Prathumsap N, Chunchai T, et al. Promoting mitochondrial fusion in doxorubicin-induced cardiotoxicity: a novel therapeutic target for cardioprotection. Clin Sci 2022;136(11):841–60.
Arya N, Singh J, Ercisli S. Anti-obesity effects of corn silk: A scoping review on its mechanism of action and future possibilities. J Food Chem Nanotechnol 2023;9(S1):S51–7.
Berrino E, Annaratone L, Detillo P, Grassini D, Bragoni A, Sapino A, et al. Tissue fixation with a formic acid-deprived formalin better preserves DNA integrity over time. Pathobiology 2023;90(3):155–65.
Brennan K, Martin K, FitzGerald S, O’sullivan J, Wu Y, Blanco A, et al. A comparison of methods for the isolation and separation of extracellular vesicles from protein and lipid particles in human serum. Sci Rep 2020;10(1):1039.
Howell D, Mayer DK, Fielding R, Eicher M, Verdonck-de Leeuw IM, Johansen C, et al. Management of cancer and health after the clinic visit: a call to action for self-management in cancer care. JNCI: J Natl Cancer Inst 2021;113(5):523–31.
Prasanna PL, Renu K, Gopalakrishnan AV. New molecular and biochemical insights of doxorubicin-induced hepatotoxicity. Life Sci 2020;250:117599.
Lin SR, Chang CH, Hsu CF, Tsai MJ, Cheng H, Leong MK, et al. Natural compounds as potential adjuvants to cancer therapy: Preclinical evidence. Br J Pharmacol 2020;177(6):1409–23.
Barakat AI, El-Nabi SH, El-Gerbed MS. Corn silk alleviates paclitaxel-induced lung toxicity in rats. J Med Life Sci 2024;6(2):182–95.
Singh P, Singh J, Rasane P, Kaur S, Nanda V, Ercisli S. Nephroprotective effect of corn silk: a review on its mechanism of action and safety evaluation. J Food Chem Nanotechnol 2023;9(S1):S28–35.
Bilgic S, Ozgocmen M. The protective effect of misoprostol against doxorubicin induced liver injury. Biotech Histochem 2019;94(8):583–91.
Belhan S, Özkaraca M, Özdek U, Kömüro?lu AU. Protective role of chrysin on doxorubicin?induced oxidative stress and DNA damage in rat testes. Andrologia 2020;52(9):e13747.
Gulati A, Singh J, Rasane P, Kaur S, Kaur J, Nanda V. Anti-cancerous effect of corn silk: a critical review on its mechanism of action and safety evaluation. 3 Biotech 2023;13(7):246.
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Copyright (c) 2025 Amber Shami, Amaidah Mir, Urfa Zaryab Mir, Hammad Ahmed Butt, Rabia Ejaz, Maria Yasmeen
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