ANGIOGENESIS ACCELERATION IN WOUND HEALING: ZINC OXIDE VERSUS BETEL LEAF EXTRACT
DOI:
https://doi.org/10.69656/pjp.v21i1.1746Keywords:
Angiogenesis, Wound repair, Zinc oxide, Betel leavesAbstract
Background: Wound healing is a complex physiological process involving haemostasis, inflammation, proliferation, and remodelling. Chronic wounds pose challenges due to delayed healing. This study compares the effects of topical zinc oxide (ZnO) and betel leaf extract on angiogenesis in wound repair. Methods: This study was carried out over 6 months from Oct 2023 to Mar 2024, at Shahida Islam Medical Complex, Lodhran. Thirty-six albino rats were divided into three groups of 12 each. A dorsal wound was created, and treatments were applied to the wound. Group A was control, and saline was applied to the wound, Group B was administered 20% ZnO on the wound, and Group C received betel leaf extract as wound dressing. Wound margin tissue was extracted, fixed, and histopathological slides were prepared. Masson trichrome stain was used to count vessels at 400× magnification under microscope. Vessel counts were analyzed via microscopy on days 3, 7, and 14. Data was analysed using one-way ANOVA. Results: On day 3, vessel counts were 4.3 for group A, 6.5 for group B, and 4.2 for group C. By day 7, counts increased to 5.8 for A, 11.54 for B, and 9.52 for C, and on day 14, they were 5.5 for A, 7.54 for B, and 6.57 for C. ZnO showed a significant increase in angiogenesis compared to control (p<0.01) and betel leaf extract (p<0.05). Conclusion: Zinc oxide accelerated wound healing more effectively than betel leaf extract.
Pak J Physiol 2025;21(1):21-4, DOI: https://doi.org/10.69656/pjp.v21i1.1746
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Copyright (c) 2025 Muhammad Ali Zubair, Suhail Marfani, Javed Mahmood, Farheen Hameed, Sama Ul Haque, Adeel Zafar
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