DIAGNOSTIC SIGNIFICANCE OF BONE MARROW TREPHINE BIOPSY IN DIFFERENT TYPES OF LYMPHOMA

Authors

  • Maham Arshad Department of Pathology, Army Medical College, National University of Medical Sciences, Rawalpindi, Pakistan
  • Aiza Saadia Department of Pathology, Army Medical College, National University of Medical Sciences, Rawalpindi, Pakistan
  • Sunila Tashfeen Department of Pathology, Army Medical College, National University of Medical Sciences, Rawalpindi, Pakistan
  • Saima Bashir Department of Pathology, Army Medical College, National University of Medical Sciences, Rawalpindi, Pakistan
  • Kehkashan Hassan Department of Pathology, Islamabad Medical and Dental College, Islamabad, Pakistan
  • Tanzeel Imran Department of Pathology, Rawal Institute of Health Sciences, Rawalpindi, Pakistan

DOI:

https://doi.org/10.69656/pjp.v21i1.1681

Keywords:

Lymphoma, Immunohistochemistry, Trephine biopsy

Abstract

Background: Bone marrow trephine biopsy is a critical diagnostic tool for detecting lymphoma. This study was conducted to evaluate the usefulness of bone marrow trephine (BMT) biopsy of lymphomas followed by immunohistochemistry. Methodology: This cross-sectional retrospective study was done at Department of Pathology (Haematology), Pak Emirates Military Hospital, Rawalpindi from Apr to Jun 2023. Through non-probability consecutive sampling, bone marrow aspiration and trephine biopsy of 43 patients were assessed to see the morphology of cells and pattern of involvement of bone marrow. Immunohistochemistry was also applied on the trephine to confirm the diagnosis and know the origin of these cells. Statistical analysis was done using SPSS-22. Quantitative data was presented as Mean±SD and qualitative data was presented as frequency and percentage. Chi-square test was applied and p?0.05 was taken as statistically significant. Results: There were 32 (74.4%) male, and 11 (25.6%) female patients. On bone marrow aspiration 16 (37.2%) had non-Hodgkin’s Lymphoma, 11 (25.6%) had Chronic Lymphocytic Leukaemia, 2 (4.7%) had diffuse Large B-Cell Lymphoma, 2 (4.7%) had Acute Lymphoblastic Leukaemia, 1 (2.3%) had T-Cell, and 2 (4.7%) showed others diagnosis like Myelodysplastic syndrome. Six (14.0%) showed inconclusive results and 3 (7.0%) had reactive bone marrow. Thirteen (30.2%) patients showed non-Hodgkin’s Lymphoma B-Cell in both aspirate and trephine, 3 (7.0%) had Chronic Lymphocytic Leukaemia, and 1 (2.3%) showed T-Cell, (p<0.001). Conclusion: Bone marrow trephine biopsy plays a pivotal role in the diagnosis of various types of lymphoma and enhances precision of diagnosis.

Pak J Physiol 2025;21(1):10–3, DOI: https://doi.org/10.69656/pjp.v21i1.1681

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Published

31-03-2025

How to Cite

1.
Arshad M, Saadia A, Tashfeen S, Bashir S, Hassan K, Imran T. DIAGNOSTIC SIGNIFICANCE OF BONE MARROW TREPHINE BIOPSY IN DIFFERENT TYPES OF LYMPHOMA. Pak J Phsyiol [Internet]. 2025 Mar. 31 [cited 2025 Apr. 24];21(1):10-3. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/1681

Issue

Vol. 21 No. 1 (2025): Pakistan Journal of Physiology

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Original Article

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Copyright (c) 2025 Maham Arshad, Aiza Saadia, Sunila Tashfeen, Saima Bashir, Kehkashan Hassan, Tanzeel Imran

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