DIAGNOSTIC SIGNIFICANCE OF BONE MARROW TREPHINE BIOPSY IN DIFFERENT TYPES OF LYMPHOMA
DOI:
https://doi.org/10.69656/pjp.v21i1.1681Keywords:
Lymphoma, Immunohistochemistry, Trephine biopsyAbstract
Background: Bone marrow trephine biopsy is a critical diagnostic tool for detecting lymphoma. This study was conducted to evaluate the usefulness of bone marrow trephine (BMT) biopsy of lymphomas followed by immunohistochemistry. Methodology: This cross-sectional retrospective study was done at Department of Pathology (Haematology), Pak Emirates Military Hospital, Rawalpindi from Apr to Jun 2023. Through non-probability consecutive sampling, bone marrow aspiration and trephine biopsy of 43 patients were assessed to see the morphology of cells and pattern of involvement of bone marrow. Immunohistochemistry was also applied on the trephine to confirm the diagnosis and know the origin of these cells. Statistical analysis was done using SPSS-22. Quantitative data was presented as Mean±SD and qualitative data was presented as frequency and percentage. Chi-square test was applied and p?0.05 was taken as statistically significant. Results: There were 32 (74.4%) male, and 11 (25.6%) female patients. On bone marrow aspiration 16 (37.2%) had non-Hodgkin’s Lymphoma, 11 (25.6%) had Chronic Lymphocytic Leukaemia, 2 (4.7%) had diffuse Large B-Cell Lymphoma, 2 (4.7%) had Acute Lymphoblastic Leukaemia, 1 (2.3%) had T-Cell, and 2 (4.7%) showed others diagnosis like Myelodysplastic syndrome. Six (14.0%) showed inconclusive results and 3 (7.0%) had reactive bone marrow. Thirteen (30.2%) patients showed non-Hodgkin’s Lymphoma B-Cell in both aspirate and trephine, 3 (7.0%) had Chronic Lymphocytic Leukaemia, and 1 (2.3%) showed T-Cell, (p<0.001). Conclusion: Bone marrow trephine biopsy plays a pivotal role in the diagnosis of various types of lymphoma and enhances precision of diagnosis.
Pak J Physiol 2025;21(1):10–3, DOI: https://doi.org/10.69656/pjp.v21i1.1681
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