EFFECTS OF AVOCADO (PERSEA AMERICANA) AQUEOUS SEED EXTRACT ON GROSS FEATURES OF LIVER AND HEPATOCYTE SIZE IN ISONIAZID AFFECTED ALBINO RATS

Abstract

Background: Isoniazid (INH) has been associated with severe hepatotoxicity and fatal liver injury. This study aimed to observe the hepatoprotective effects of concomitant use of avocado aqueous seed extract on the gross features of liver and size of hepatocytes in INH induced hepatotoxicity in albino rats. Methods: This experimental study was conducted from Jan–Jun 2019. Thirty-six male albino rats were divided into 4 groups. Groups were administered treatment orally for 30 days. Group 1 received distilled water 1 ml/Kg/day. Group 2 received INH 100 mg/Kg/day dissolved in 1 ml distilled water. Group 3 was given aqueous avocado seed extract 250 mg/Kg/day in 2 ml distilled water and Group 4 received aqueous avocado seed extract 500 mg/Kg/day in 4 ml distilled water. Results: Highest mean liver weight was seen in Group 4 and lowest in Group 2. The weight of liver was significantly different among all groups but Relative Tissue Weight Index among all groups was insignificant. Colour of liver in four groups varied from reddish to pale red and reddish brown. The texture of all livers was smooth. Hepatocyte size was found to be largest in Group 2 and smallest in Group 1. Comparison among groups showed significant difference in sizes of hepatocytes. Conclusion: Concomitant use of aqueous avocado seed extract prevents INH-induced hepatotoxicity by maintaining body and liver weights, gross features of liver, and size of hepatocytes in a dose-dependent manner. 

Pak J Physiol 2022;18(2):38–42