PREVALENCE OF HEPATITIS B VIRUS IN THE EMPLOYEES OF UNIVERSITY OF AGRICULTURE, PESHAWAR
DOI:
https://doi.org/10.69656/pjp.v13i3.72Keywords:
HBV, ICT, PCR, Vaccination, Screening, HBsAgAbstract
Objectives: To detect the presence of Hepatitis B virus (HBV) in the employees of the University of Agriculture, Peshawar. Method: Two hundred employees (180 males and 20 females) of the University were selected on the given criteria. Blood samples were collected and serum was separated by centrifuging the samples at 5,000 rpm for 8 minutes. The sera were transferred to eppendorf tubes and stored at -20 °C. Samples were initially screened for Hepatitis B surface Antigen (HBsAg) by Immuno Chromatographic Test (ICT). All the serum samples were used for HBV DNA extraction and subsequent two steps Polymerase Chain Reaction (PCR) assay developed at the Institute of Biotechnology and Genetic Engineering (IBGE) for the confirmation of active infection of HBV. PCR products were detected on 2% Agarose gel pre-stained with Ethedium Bromide. Results: Results of ICT indicated that 6 (3%) were anti HBsAg hepatitis positive, of which 5 (2.5%) were males and 1 (0.5%) was female. The PCR results confirmed that 4 (2%) were actively infected, of which 3 (1.5%) were male and 1 (0.5%) was female. The results indicated that infection with HBV was more in male than female and was more in the age group of 25–35 years. Conclusion: The infection with HBV in the employees of the University of Agriculture, Peshawar was 3% as determined by ICT method and 2%. It was more in male than female and was more in younger age than older age. Screening and vaccination of the employees is recommended.
Pak J Physiol 2017;13(3):12–4
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2. Bhatti FA, Ullah Z, Salamat N, Ayub M, Ghani E. Anti-hepatitis B core antigen testing, viral markers, and occult hepatitis B virus infection in Pakistani blood donors: implications for transfusion practice. Transfusion 2007;47:74.
3. Khan N, Ahmed SM, Khalid MM, Siddiqui SH, Merchant AA. Effect of gender and age on the knowledge, attitude and practice regarding hepatitis B and C and vaccination status of hepatitis B among medical students of Karachi, Pakistan. J Pak Med Assoc 2010;60:450–5.
4. Aziz S, Khanani R, Noorulain W, Rajper J. Hepatitis B and C in rural and periurban Sindh. J Pak Med Assoc 2010;60:853–7.
5. The Nation. To raise awareness about the menace and the measures to prevent hepatitis (special article) on The World Hepatitis Day. The Nation July 7, 2014.
6. Basit A, Rahim K, Ahmad I, Shafiq M, Mushtaq S, Shaheen H, et al. Prevalence of Hepatitis B and C infection in Pakistan. J Infect Mol Biol 2014;2(3):35–8.
7. Younis M, Hussain AB, Rafique T, Iqbal H. Frequency of Hepatitis B and C in young a symptomatic males. Pak Armed Forces Med J 2013;63(2):245–8.
8. Majid A, Khan MS, Ullah S. Rising prevalence of Hepatitis B and C and risk factors at District Headquarter Teaching Hospital Bannu, Khyber Pakhtunkhwa. J Coll Physicians Surg Pak 2010;20(7):492–3.
9. Hayder I, Ahmad W, Alam SE. Comparison of different ICT kits for HBÂAg and anti-HCV using gold standard ELISA. Pak J Med Res 2012;51(3):72–6.
10. Khan ZA, Aslam MI, Ali S. The frequency of hepatitis B and C among volunteer blood donors in Balochistan. Hepat Mon 2007;7(2):73–6.
11. Khan F, Shams S, Qureshi ID, Israr M, Khan H, Sarwar MT, et al. Hepatitis B virus infection among different sex and age groups in Pakistani Punjab. Virol J 2011;8:225.
12. Alan MM, Zaidi SZ, Malik SA, Shaukat S, Naeem A, Sharif S, et al. Molecular epidemiology of hepatitis B virus genotypes in Pakistan. BMC Infect Dis 2007;7:115.
13. Cisneros-Castolo M, Hernández-Ruiz L, Ibarra-Robles IE, Fernández-Gárate RH, Escobedo-De La Peña J. Prevalence of Hepatitis B Virus Infection and related risk factors in a rural community of Mexico. Am J Trop Med Hyg 2001;65:759–63.
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Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, projected, cited and quoted with full reference of, and acknowledgement to the author(s) and the PJP. The contents are published with an international CC-BY-ND-4.0 License.
