EFFECT OF TRIBULUS TERRESTRIS ON SERUM LUTEINIZING HORMONE IN SPRAGUE DAWLEY RATS

Authors

  • Sidra Hamid Department of Physiology, Rawalpindi Medical University, Rawalpindi, Pakistan.
  • Aneela Jamil Department of Biochemistry, Foundation University Medical College, Islamabad, Pakistan
  • Aneeqa Rashid Department of Physiology, Hitec Institute of Medical Sciences, Taxila, Pakistan
  • Qaiser Aziz Department of Medicine, Holy Family Hospital, Rawalpindi, Pakistan
  • Muhammad Aslam National University of Medical Sciences, Rawalpindi, Pakistan

DOI:

https://doi.org/10.69656/pjp.v13i4.287

Keywords:

luteinizing hormone (LH), Serum, Tribulus terrestris

Abstract

Background: Tribulus terrestris is a creeping herb that has been used in the past for treating impotence. The extract obtained from Tribulus mainly contains the active component protodioscin. Administration of the extract improves libido and spermatogenesis. Protodioscin increases the levels of luteinizing hormone (LH), testosterone, dehydroepiandrosterone (DHEA) and dihydrotestosterone (DHT). The objective of present study was to determine the effect of Tribulus terrestris on serum luteinizing hormone in male Sprague Dawley rats. Methods: The study was carried on 60 adult male Sprague Dawley rats aged 90–120 days and with average body weight of 200±50 grams. Rats were divided into two groups with 30 rats per group. Group A received 0.5 ml distilled water once daily orally. Group B was administered orally Tribulus aqueous extract 6 mg/Kg once daily. All the doses were given to rats for 8 weeks. After 8 weeks, serum luteinizing hormone was measured in both groups. The mean values of LH were compared using SPSS. Alpha value was kept at 0.05. Results: The mean LH level was 1.26±0.54 for control group while it was 1.75±0.65 for the experimental group. The difference between control group and experimental group was statistically significant (p<0.001). Conclusion: Tribulus terrestris increases the levels of LH when administered in appropriate doses.

Pak J Physiol 2017;13(4):38–40

Downloads

Download data is not yet available.

References

1. Sharma I, Parashar B, Dhamija HK, Sharma R. An Ayurvedic Arena for Hypertension Treatment. Asian J Pharma Res 2012;2(2):54–8.
2. Kotta S, Ansari SH, Ali J. Exploring scientifically proven herbal aphrodisiacs. Pharmacogn Rev 2013;7(13):1–10.
3. Kamboj P Aggarwal M, Puri S, Singla SK. Effect of aqueous extract of Tribulus terrestris on oxalate-induced oxidative stress in rats. Indian J Nephrol 2011;21(3):154–9.
4. Sharifi AM, Darabi R, Akbarloo N. Study of antihypertensive mechanism of Tribulus terrestris in 2K1C hypertensive rats: role of tissue ACE activity. Life Sci 2003;73(23):2963–71.
5. Singh S, Nair V, Gupta YK. Evaluation of the aphrodisiac activity of Tribulus terrestris Linn in sexually sluggish male albino rats. J Pharmacol Pharmacother 2012;3(1):43–7.
6. Al-Ali M, Wahbi S, Twaij H, Al-Badr A. Tribulus terrestris: Preliminary study of its diuretic and contractile effects and comparison with Zea mays. J Ethnopharmacol 2003;85:257–60.
7. Jashni HK, Shiravani SM, Hoshmand F. The effect of the Tribulus terrestris extract on spermatogenesis in the rat. J Jahrom Univ Med Sci 2012;9(4):8–13.
8. Dinchev D, Janda B, Evstatievac L, Oleszek W, Aslani MR, Kostova I. Distribution of steroidal saponins in Tribulus terrestris from different geographical regions. Phytochemistry 2008;69(1):176–86.
9. Rawat AKS Srivastava A, Tiwari SS, Srivastava S. Quantification of protodioscin and prototribestin in fruits of Tribulus terrestris L. collected from different phyto-geographical zones of India. J Liq Chromatogr Relat Technol 2013;36:1810–21.
10. Gauthaman K, Ganesan AP, Prasad R. Sexual effects of Puncturevine (Tribulus terrestris) extract (Protodioscin): An evaluation using a rat model. J Altern Complement Med 2003;9:257–65.
11. Cek S, Turan F, Atik E. The effects of Gokshura, Tribulus terrestris on sex reversal of guppy, Poecilia reticulata. Pak J Biol Sci 2007;10(5):718–25.
12. Sellandi TM, Thakar AB, Baghel MS. Clinical study of Tribulus terrestris Linn. in oligozoospermia: A double blind study. Ayu 2012;33(3):356–64.
13. Dehghan A, Esfandiari A, Bigdeli SM. Alternative treatment of ovarian cysts with Tribulus terrestris extract: a rat model. Reprod Domest Anim 2012;47(1):e12–5.
14. Gauthaman K, Ganesan AP. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction, an evaluation using primates, rabbit and rat. Phytomedicine 2008;15(1-2):44–54.
15. Isidori AM, Pozza C, Gianfrilli D, Isidori A. Medical treatment to improve sperm quality. Reprod Biomed Online 2006;12(6):704–14.
16. Morakinyo AO, Iranloye BO, Daramola AO, Adegoke OA. Antifertility effect of calcium channel blockers on male rats: association with oxidative stress. Adv Med Sci 2011;56(1):95–105.
17. Shalaby MA, Hammouda AA. Assessment of protective and anti-oxidant properties of Tribulus terrestris fruits against testicular toxicity in rats. J Intercult Ethnopharmacol 2014;3(3):113–8.
18. Moghaddam MH, Khalili M, Maleki M, Abadi ME. The effect of oral feeding of Tribulus terrestris L. on sex hormone and gonadotropin levels in addicted male rats. Int J Fertility Sterility 2013;7(1):57.
19. El-Tantawy WH, Temraz A, El-Gindi OD. Free serum testosterone level in male rats treated with Tribulus alatus extracts. Int Braz J Urol 2007;33(4):554–8; discussion 8–9.
20. Neychev VK, Mitev VI. The aphrodisiac herb Tribulus terrestris does not influence the androgen production in young men. J Ethnopharmacol 2005;101(1-3):319–23.
21. Pokrywka A, Obmiński Z, Malczewska-Lenczowska J, Fijałek Z, Turek-Lepa E, Grucza R. Insights into Supplements with Tribulus terrestris used by Athletes. J Hum Kinet 2014;41:99–105.
22. Santos CA Jr, Reis LO, Destro-Saade R, Luiza-Reis A, Fregonesi A. Tribulus terrestris versus placebo in the treatment of erectile dysfunction: A prospective, randomized, double blind study. Actas Urol Esp 2014;38(4):244–8.
23. Iacono F, Prezioso D, Illiano E, Romeo G, Ruffo A, Amato B. Sexual asthenia: Tradamixina versus Tadalafil 5 mg daily. BMC Surg 2012;12(Suppl 1):S23. doi:10.1186/1471-2482-12-S1-S23.

Downloads

Published

31-12-2017

How to Cite

1.
Hamid S, Jamil A, Rashid A, Aziz Q, Aslam M. EFFECT OF TRIBULUS TERRESTRIS ON SERUM LUTEINIZING HORMONE IN SPRAGUE DAWLEY RATS. Pak J Phsyiol [Internet]. 2017 Dec. 31 [cited 2024 Oct. 8];13(4):38-40. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/287