EFFECTS OF LONG-TERM ITOPRIDE HYDROCHLORIDE TREATMENT ON RAT PROXIMAL CONVOLUTED TUBULES AND SERUM GLUTATHIONE LEVELS
DOI:
https://doi.org/10.69656/pjp.v21i4.1768Keywords:
Functional dyspepsia, Glutathione, Luminal diameter, Proximal convoluted tubuleAbstract
Background: Functional dyspepsia is a chronic gastric motility disorder marked by upper gastrointestinal symptoms; itopride hydrochloride, a prokinetic benzamide derivative acting via acetylcholinesterase and dopamine D? receptor inhibition, is widely used, yet evidence on its long-term systemic effects remains limited. Objective of this study was to evaluate the effects of long-term administration of itopride hydrochloride on renal histomorphology and serum glutathione levels in male Wistar albino rats. Methods: This laboratory-based experimental study was conducted in Anatomy Department, CMH Multan Institute of Medical Sciences, Pakistan. Sixty adult male Wistar albino rats were divided into control and experimental groups (n=30 each). Controls received distilled water, while the experimental group received itopride hydrochloride (4.4 mg/Kg) orally three times a day for 15 days. Kidneys were processed, paraffin embedded, and stained with haematoxylin and eosin. Proximal convoluted tubule (PCT) height and luminal diameter were measured using image. Serum glutathione was assessed with ELISA. Data were analyzed using Student’s t-test. Results: The experimental group showed a significant reduction in PCT epithelial height and a significant increase in luminal diameter compared to controls (p=0.001). Serum glutathione levels were also significantly decreased in experimental groups as compared to controls (p=0.001). Conclusion: Long-term administration of itopride hydrochloride adversely affects renal tubular histomorphology and reduces serum glutathione levels in Wistar albino rats.
Pak J Physiol 2025;21(4):3–6, DOI: https://doi.org/10.69656/pjp.v21i4.1768
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Copyright (c) 2025 Aasma Hashmi; Hamd Binte Shahab Syed, Muhammad Ali Rabbani, Ayesha Irfan, Zoha Hashmi
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