AMELIORATIVE EFFECTS OF VITAMIN E ON CARBAMAZEPINE INDUCED HEPATIC TOXICITY IN MALE SPRAGUE DAWLEY RATS
DOI:
https://doi.org/10.69656/pjp.v20i4.1721Keywords:
Carbamazepine, Vitamin E, hepatic injury, liver enzymes, liver weightAbstract
Background: Carbamazepine (CBZ) is prescribed for various neurological and psychiatric disorders. While its therapeutic efficacy is well-established, concerns about hepatotoxicity have arisen due to liver enzyme abnormalities and rare cases of severe liver injury. The aim of this study was to investigates protective effects of Vitamin E against carbamazepine-induced hepatotoxicity via liver enzyme analysis in male Sprague Dawley rats. Methods: Fifty-four male albino Sprague Dawley rats were randomly divided into Groups A, B, and C. Each group was further split into three subgroups of six, based on treatment duration (2, 4, and 6 weeks). Group A served as the control group. Group B received Carbamazepine (50 mg/Kg) via gastric gavage for 2, 4, or 6 weeks. Group C received Vitamin E (200 mg/Kg/day) along with CBZ for the same periods. After the respective treatment durations, the animals were weighed and sacrificed, and blood was collected via cardiac puncture for liver enzyme analysis. Results: Carbamazepine caused hepatotoxicity, evidenced by decreased body weight, increased liver weight, and elevated ALT, ALP, and GGT levels compared to controls. Co-administration of Vitamin E significantly reduced body weight loss, liver weight gain, and the rise in liver enzymes. Conclusion: The findings of this study suggest the potential protective role of Vitamin E against CBZ-induced hepatotoxicity in rat model. Vitamin E may aid in protecting the adverse reactions of CBZ as an adjuvant therapy.
Pak J Physiol 2024;20(4):56-9, Doi: https://doi.org/10.69656/pjp.v20i4.1721
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References
Wang L, Wang Y, Zhang RY, Wang Y, Liang W, Li TG. Management of acute carbamazepine poisoning: A narrative review. World J Psychiatry 2023;13(11):816–30.
Osuntokun OS, Babatunde AA, Olayiwola G, Atere TG, Oladokun OO, Adedokun KI. Assessment of the biomarkers of hepatotoxicity following carbamazepine, levetiracetam, and carbamazepine-levetiracetam adjunctive treatment in male Wistar rats. Toxicol Rep 2021;8:592–8.
Bai Z, Jia K, Chen G, Liao X, Cao Z, Zhao Y, et al. Carbamazepine induces hepatotoxicity in zebrafish by inhibition of the Wnt/?-catenin signaling pathway. Environ Pollut 2021;276:116688.
Kareem DA, Sadoon AH, Majeed MF, Abbas BA. Histological and enzymatic response of carbamazepine-induced liver injury as a biomarker in male mice. Iran J War Pub Health 2022;14(4):367–75.
Rani J, Dhull SB, Rose PK, Kidwai MK. Drug-induced liver injury and anti-hepatotoxic effect of herbal compounds: a metabolic mechanism perspective. Phytomedicine 2024;122:155142.
Li X, Tang J, Mao Y. Incidence and risk factors of drug?induced liver injury. Liver Int 2022;42(9):1999–2014.
Rai M, Paudel N, Sakhrie M, Gemmati D, Khan IA, Tisato V, et al. Perspective on perspective on quantitative structure-toxicity relationship (QSTR) models to predict hepatic biotransformation of xenobiotics. Livers 2023;3(3):448–62.
Alahmadi BA, El-Alfy SH, Hemaid AM, Abdel-Nabi IM. The protective effects of vitamin E against selenium-induced oxidative damage and hepatotoxicity in rats. J Taibah Univ Sci 2020;14(1):709–20.
Pahrudin Arrozi A, Shukri SNS, Wan Ngah WZ, Mohd Yusof YA, Ahmad Damanhuri MH, Jaafar F, et al. Comparative effects of alpha-and gamma-tocopherol on mitochondrial functions in Alzheimer’s disease in vitro model. Sci Rep 2020;10(1):8962.
Ungurianu A, Zanfirescu A, Ni?ulescu G, Margin? DJA. Vitamin E beyond its antioxidant label. Antioxidants 2021;10(5):634.
Ibrahim MA, Ibrahim HM, Mohamed AA, Tammam HG. Vitamin E supplementation ameliorates the hepatotoxicity induced by Tramadol: Toxicological, histological and immunohistochemical study. Toxicol Mech Methods 2020;30(3):177–88.
Arifin WN, Zahiruddin WM. Sample size calculation in animal studies using resource equation approach. Malays J Med Sci 2017;24(5):101–5.
Saadh MJ, Haddad M, Dababneh MF, Bayan MF, Al-Jaidi BA. A guide for estimating the maximum safe starting dose and conversion it between animals and humans. Syst Rev Pharm 2020;11(4):98–101.
Enye LA, Saka OS, Adekanye UA, Adekeye AO, Abijo AZ, Ige MS. Hippocampal histomorphology and biochemistry in Rats fed diets containing carbamazepine. African J Biomed Res 2022;25(1):89–94.
Ali AB, Younus N, Mukhtar S, Faisal L, Kazmi T, Atif A. Evaluating vitamin E’s antioxidative effect on carbamazepine-induced spleen damage in rat models. Pak J Med Dent 2024;13(3):132–9.
Fang J, Xie S, Chen Z, Wang F, Chen K, Zuo Z, et al. Protective effect of vitamin E on cadmium-induced renal oxidative damage and apoptosis in rats. Biol Trace Elem Res 2021;199(12):4675–87. doi: 10.1007/s12011-021-02606-4
Samare-Najaf M, Zal F, Safari S. Primary and secondary markers of doxorubicin-induced female infertility and the alleviative properties of quercetin and vitamin E in a rat model. Reprod Toxicol 2020;96:316–26.
Jaber NR, Al-Bakri NA. The morphological and histopathological liver abnormalities caused by carbamazepine-induced injury in female albino mice. J Biotech Res Center 2024;18(1):105–18.
Bai Y, Li T, Liu J, Wang Y, Wang C, Ju S, et al. Aerobic exercise and vitamin E improve high-fat diet-induced NAFLD in rats by regulating the AMPK pathway and oxidative stress. Eur J Nutr 2023;62(6):2621–32.
Aliyu M, Saleh M, Alhassan A, Zuberu J, Adamu B, Iliya B, et al. Evaluation of the effect of co-administration of resveratrol and vitamin E on carbamazepine-induced oxidative stress in male adult Wistar rats. J Afr Assoc Physiol Sci 2017;5(2):93–100.
George J, Lu Y, Tsuchishima M, Tsutsumi M. Cellular and molecular mechanisms of hepatic ischemia-reperfusion injury: The role of oxidative stress and therapeutic approaches. Redox Biol 2024;75:103258.
Al-Shaikh TM. Ameliorating effect of vitamin E on liver damage caused by administering tartrazine in male mice. Asian J Pharm Res Health Care 2021;13(1):61–9.
Ramezani V, Tavakoli F, Emami A, Javadi SA. Comparison of the hepatotoxicity of carbamazepine, sodium valproate, phenytoin, lamotrigine, and vigabatrin in a rat model. Avicenna J Pharm Res 2022;3(1):17–22.
Satué K, Miguel-Pastor L, Chicharro D, Gardón JC. Hepatic enzyme profile in horses. Animals 2022;12(7):861.
Nwangwa JN, Udefa AL, Obeten CE, Obi PM, Adie PU, Kanu UJ. Vitamin E supplementation counters the hepatotoxic effects of tramadol on male Wistar rats. IOSR J Dent Med Sci 2019;18(6):74–86.
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