PROTECTIVE ROLE OF SOYBEAN OIL ON LETROZOLE INDUCED MORPHOLOGICAL CHANGES IN THE TRABECULAR DIAMETER OF LONG BONE: A MOUSE MODEL
DOI:
https://doi.org/10.69656/pjp.v20i2.1647Keywords:
Letrozole, Soy bean oil, Osteoporosis, femur, Pakistan, CancerAbstract
Background: Letrozole, a commonly prescribed neoadjuvant chemotherapeutic drug in hormone receptor-positive breast cancer females, causes osteoporosis along with several other debilitating effects. Objective of this study was to evaluate the protective role of soybean oil against letrozole-induced histo-morphological changes in femur of mice. Methods: This laboratory based experimental study was carried out in animal house at Pakistan Council of Scientific and Industrial Research Centre (PCSIR) Peshawar and Khyber Medical University (KMU). Sixty female albino mice were divided into control (group-A) and experimental groups (B and C respectively). The experimental group B was given Letrozole drug at a dose of 1 mg/Kg/day for 28 days, and Experimental group C was given both Letrozole drug at a dose of 1 mg/Kg/day and 1 ml 10% Soybean oil. The mice were weighed. The animals were sacrificed and femurs were dissected and processed to find out changes in its gross morphology, weight, and trabecular diameter. Trabecular diameter was measured microscopically by using ocular graticule and stage micrometer. Data were analyzed using SPSS-22. Mean and standard deviation were calculated for weight of mice, weight of femur and trabecular diameter. Independent sample t-test was applied to compare the continuous variable among two groups. Results: The weight of mice, weight of femur and trabecular diameter of femur in mice of Experimental group-C (Soy bean oil) was greater than Experimental group-B (Letrozole) but lesser than control group-A. Conclusion: Soybean oil has protective role against letrozole induces bone loss in female albino mice.
Pak J Physiol 2024;20(2):46-9
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