PROTECTIVE ROLE OF SOYBEAN OIL ON LETROZOLE INDUCED MORPHOLOGICAL CHANGES IN THE TRABECULAR DIAMETER OF LONG BONE: A MOUSE MODEL
DOI:
https://doi.org/10.69656/pjp.v20i2.1647Keywords:
Letrozole, Soy bean oil, Osteoporosis, femur, Pakistan, CancerAbstract
Background: Letrozole, a commonly prescribed neoadjuvant chemotherapeutic drug in hormone receptor-positive breast cancer females, causes osteoporosis along with several other debilitating effects. Objective of this study was to evaluate the protective role of soybean oil against letrozole-induced histo-morphological changes in femur of mice. Methods: This laboratory based experimental study was carried out in animal house at Pakistan Council of Scientific and Industrial Research Centre (PCSIR) Peshawar and Khyber Medical University (KMU). Sixty female albino mice were divided into control (group-A) and experimental groups (B and C respectively). The experimental group B was given Letrozole drug at a dose of 1 mg/Kg/day for 28 days, and Experimental group C was given both Letrozole drug at a dose of 1 mg/Kg/day and 1 ml 10% Soybean oil. The mice were weighed. The animals were sacrificed and femurs were dissected and processed to find out changes in its gross morphology, weight, and trabecular diameter. Trabecular diameter was measured microscopically by using ocular graticule and stage micrometer. Data were analyzed using SPSS-22. Mean and standard deviation were calculated for weight of mice, weight of femur and trabecular diameter. Independent sample t-test was applied to compare the continuous variable among two groups. Results: The weight of mice, weight of femur and trabecular diameter of femur in mice of Experimental group-C (Soy bean oil) was greater than Experimental group-B (Letrozole) but lesser than control group-A. Conclusion: Soybean oil has protective role against letrozole induces bone loss in female albino mice.
Pak J Physiol 2024;20(2):46-9
Downloads
References
Sudhakar A. History of cancer, ancient and modern treatment methods. J Cancer Sci Ther 2009;1(2):1–4.
Li C, Uribe D, Daling J. Clinical characteristics of different histologic types of breast cancer. Br J Cancer 2005;93(9):1046–52.
Ferlay J, Héry C, Autier P, Sankaranarayanan R. Global burden of breast cancer. In: Li C, (Ed). Breast Cancer Epidemiology: Springer; 2010. p. 1–19.
Waks AG, Winer EP. Breast cancer treatment: A review. JAMA 2019;321(3):288–300.
Khan NH, Duan SF, Wu DD, Ji XY. Better reporting and awareness campaigns needed for breast cancer in Pakistani women. Cancer Mang Res 2021;13:2125–29.
Hefti MM, Hu R, Knoblauch NW, Collins LC, Haibe-Kains B, Tamimi RM, et al. Estrogen receptor negative/progesterone receptor positive breast cancer is not a reproducible subtype. Breast Cancer Res 2013;15(4):R68.
Colditz GA. Epidemiology and prevention of breast cancer. Cancer Epidemiol Biomarkers Prev 2005;14(4):768–72.
Maughan KL, Lutterbie MA, Ham PS. Treatment of breast cancer. Am Fam Physician 2010;81(11):1339–46.
Sun YS, Zhao Z, Yang ZN, Xu F, Lu HJ, Zhu ZY, et al. Risk factors and preventions of breast cancer. Int J Biol Sci 2017;13:1387–97.
Amer SA, Smith J, Mahran A, Fox P, Fakis A. Double-blind randomized controlled trial of letrozole versus clomiphene citrate in subfertile women with polycystic ovarian syndrome. Hum Reprod 2017;32(8):1631–8.
Requena A, Herrero J, Landeras J, Navarro E, Neyro JL, Salvador C, et al. Use of letrozole in assisted reproduction: a systematic review and meta-analysis. Hum Reprod Update 2008;14:571–82.
Zaman K, Thürlimann B, Huober J, Schönenberger A, Pagani O, Lüthi J, et al. Bone mineral density in breast cancer patients treated with adjuvant letrozole, tamoxifen, or sequences of letrozole and tamoxifen in the BIG 1-98 study (SAKK 21/07). Ann Oncol 2011;23(6):1474–81.
Yonden Z, Aydin M, Alcin E, Kelestemur MH, Kutlu S, Yilmaz B. Effects of letrozole on bone biomarkers and femur fracture in female rats. J Physiol Biochem 2009;65(3):267–75.
Barrett JR. The science of soy: what do we really know? Environ Health Perspect 2006;114(6):A352–8.
Messina M. Soy and health update: evaluation of the clinical and epidemiologic literature. Nutrients 2016;8(12):754.
Setchell KD, Cassidy A. Dietary isoflavones: biological effects and relevance to human health. J Nutr 1999;129(3):758S–767S.
Xiao CW. Health effects of soy protein and isoflavones in humans. J Nutr 2008;138(6):1244S–1249S.
Anwar N, Hamid S, Butt SA. Effect of Nigella sativa on number of cystic follicles in letrozole induced polycystic ovaries in mice: Nigella Sativa & Polycystic Ovaries in Mice. Pak Armed Forces Med J 2016;66(3):310–13.
Clark JD, Gebhart GF, Gonder JC, Keeling ME, Kohn DF. The 1996 guide for the care and use of laboratory animals. ILAR J 1997;38(1):41–8.
Guide for the care and use of laboratory animals. Washington, DC: Institute of Laboratory Animal Resources Commission on Life Sciences, National Research Council, National Academy Press, 1985.
Kalam A, Talegaonkar S, Vohora D. Effect of aromatase inhibitors on bone turnover markers in vinyl cyclohexene diepoxide treated ovotoxic female mice. Preced Br Pharmacol Soc 2016.
Rachner TD, Göbel A, Jaschke NP, Hofbauer LC. Challenges in preventing bone loss induced by aromatase inhibitors. J Clin Endocrinol Metab 2020;105(10):3122–33.
Marcu F, Bogdan F, Mutiu G, Lazar L. The histopathological study of osteoporosis. Rom J Morphol Embryol 2011;52(1 Suppl):321–5.
Pilutin A, Misiakiewicz-Has K, Kolasa-Wo?osiuk A, Trybek G, Urban F, Marchlewicz M, et al. Morphology and serum and bone tissue calcium and magnesium concentrations in the bones of male rats chronically treated with letrozole, a nonsteroidal cytochrome P450 aromatase inhibitor. Connect Tissue Res 2021;62(4):454–63.
Gaillard S, Stearns V. Aromatase inhibitor-associated bone and musculoskeletal effects: new evidence defining etiology and strategies for management. Breast Cancer Res 2011;13(2):205.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, projected, cited and quoted with full reference of, and acknowledgement to the author(s) and the PJP. The contents are published with an international CC-BY-ND-4.0 License.