EFFECTS OF L-CARNITINE THERAPY ON FAT AND GLUCOSE METABOLISM AMONG MELDONIUM INDUCED CARNITINE DEPLETION IN ALBINO WISTAR RATS

Authors

  • Sumayya Qazi Department of Biochemistry, Isra University, Hyderabad, Pakistan.
  • Mumtaz Ali Qureshi Department of Biochemistry, Isra University, Hyderabad, Pakistan.
  • Abroo Fatima Qazi Department of Physiology, Isra University, Hyderabad, Pakistan.
  • Amin Fahim Peoples University of Medical and Health Sciences For Women, Nawabshah, Pakistan
  • Ghulam Shah Nizamani Department of Pathology, Isra University, Hyderabad, Pakistan
  • Bakhtawar Qureshi Department of Community Medicine, Isra University, Hyderabad, Pakistan

Keywords:

Blood glucose, Carnitine, Cholesterol, Lipoproteins, HDL, LDL

Abstract

Background: L-carnitine therapy has a potential role in lipid metabolism and glucose homeostasis. Despite its promising effects, further investigation is warranted. This study aimed to determine the impact of L-carnitine therapy of low and high doses on fat and glucose metabolism of albino Wistar rats after Meldonium induced carnitine depletion. Methods: A total number of 48 albino Wistar rats were recruited and divided into four groups 12 rats in each group. The bedding material was changed every second day and all animals were given Kaytee Supreme Fortified Daily Diet rat food and clean distal water ad libitum at room temperature. The experiment was commenced after 10 days of acclimatization. Results: Both high (300 mg/Kg) and low-dose (500 mg/Kg) L-carnitine significantly improved (p<0.05) blood glucose levels, TC, LDL, and HDL after four weeks of L-carnitine therapy. However, high-dose therapy showed significantly higher HDL improvements and decreased TC and LDL levels than low-dose therapy (p<0.05) among Meldonium carnitine-depleted rats. Conclusion: L-carnitine high dose therapy (500 mg/Kg of body weight) had a more potent effect on lipid profile in comparison to low dose (300 mg/Kg of body weight).

Pak J Physiol 2024;20(1):26-9

Downloads

Download data is not yet available.

References

Thakur Y, Bharti R, Sharma R. Myths and reality of L-carnitine (3-Hydroxy 4-N trimethylammonium butyrate) supplementation and its chemistry: A systematic review. SCPINM-2021. Materials Today: Proceedings 2022;48:1277–82.

Delgado CA, Balbueno Guerreiro GB, Diaz Jacques CE, de Moura Coelho D, Sitta A, Manfredini V, et al. Prevention by L-carnitine of DNA damage induced by 3-hydroxy-3-methylglutaric and 3-methylglutaric acids and experimental evidence of lipid and DNA damage in patients with 3-hydroxy-3-methylglutaric aciduria. Arch Biochem Biophys 2019;668:16–22.

Yamada K, Taketani T. Management and diagnosis of mitochondrial fatty acid oxidation disorders: focus on very-long-chain acyL-CoA dehydrogenase deficiency. J Hum Genet 2019;64(2):73–85.

Macchione F, Salviati L, Bordugo A, Vincenzi M, Camilot M, Teofoli F, et al. Multiple acyl-COA dehydrogenase deficiency in elderly carriers. J Neurol 2020;267(5):1414–9.

EL-Sheikh HM, EL-Haggar SM, Elbedewy TA. Comparative study to evaluate the effect of L-carnitine plus glimepiride versus glimepiride alone on insulin resistance in type 2 diabetic patients. Diabetes Metab Syndr 2019;13(1):167–73.

Sangouni AA, Pakravanfar F, Ghadiri-Anari A, Nadjarzadeh A, Fallahzadeh H, Hosseinzadeh M. The effect of L-carnitine supplementation on insulin resistance, sex hormone-binding globulin and lipid profile in overweight/obese women with polycystic ovary syndrome: A randomized clinical trial. Eur J Nutr 2022;61(3):1199–207.

Li LY, Limbu SM, Ma Q, Chen LQ, Zhang ML, Du ZY. The metabolic regulation of dietary L?carnitine in aquaculture nutrition: Present status and future research strategies. Rev Aquac 2019 Nov;11(4):1228–57.

Bene J, Hadzsiev K, Melegh B. Role of carnitine and its derivatives in the development and management of type 2 diabetes. Nutr Diabetes 2018;8(1):8.

Li N, Zhao H. Role of carnitine in non-alcoholic fatty liver disease and other related diseases: An update. Front Med (Lausanne) 2021;8:689042.

Gao X, Tian Y, Randell E, Zhou H, Sun G. Unfavorable associations between serum trimethylamine N-oxide and L-carnitine levels with components of metabolic syndrome in the new foundland population. Front Endocrinol (Lausanne) 2019;10:168.

Wang W, Bai M, Jiang T, Li C, Li P, Zhou H, et al. Clozapine-induced reduction of L-carnitine reabsorption via inhibition/down-regulation of renal carnitine/organic cation transporter 2 contributes to liver lipid metabolic disorder in mice. Toxicol Appl Pharmacol 2019;363:47–56.

Chen YC, Cheng CY, Liu CT, Sue YM, Chen TH, Hsu YH, et al. Combined protective effects of oligo-fucoidan, fucoxanthin, and L-carnitine on the kidneys of chronic kidney disease mice. Eur J Pharmacol 2021;892:173708.

Agarwal A, Sengupta P, Durairajanayagam D. Role of L-carnitine in female infertility. Reprod Biol Endocrinol 2018;16(1):5.

Beshir SA, Zannoun MA, El Samanoudy MI, AL-Samee SH. Evaluation of the Effect of L-Carnitine Supplementation in Preterm Neonates Suffering from Respiratory Distress Syndrome. Int J Med Arts 2022;4(6):2400–6.

Kim MK, Park JK, Paek SK, Kim JW, Kwak IP, Lee HJ, et al. Effects and pregnancy outcomes of L?carnitine supplementation in culture media for human embryo development from in vitro fertilization. J Obstet Gynaecol Res 2018;44(11):2059–66.

Thiagarajan P, Chalmers J, Ban L, Grindlay D, Aithal GP. L-carnitine supplementation in non-alcoholic fatty liver disease: A systematic review and meta-analysis. World J Meta-Anal 2020;8(1):4–14.

Virmani MA, Cirulli M. The Role of L-Carnitine in Mitochondria, Prevention of Metabolic Inflexibility and Disease Initiation. Int J Mol Sci 2022;23(5):2717.

Salic K, Gart E, Seidel F, Verschuren L, Caspers M, van Duyvenvoorde W, et al. Combined treatment with L-carnitine and nicotinamide riboside improves hepatic metabolism and attenuates obesity and liver steatosis. Int J Mol Sci 2019;20(18):4359.

Ko J, Wong EY, Tran HN, Tran RJ, Cao DX. The glycemic, cholesterol, and weight effects of L-carnitine in diabetes: a systematic review and meta-analysis of randomized controlled trials. Diabetes Epidemiol Manage 2022;10(1):100122.

Hamedi-Kalajahi F, Imani H, Mojtahedi S, Shabbidar S. Effect of L-carnitine supplementation on inflammatory markers and serum glucose in hemodialysis children: a randomized, placebo-controlled clinical trial. J Ren Nutr 2022;32(2):144–51.

Lin TJ, Tang SC, Liao PY, Dongoran RA, Yang JH, Liu CH. A comparison of L-carnitine and several cardiovascular-related biomarkers between healthy vegetarians and omnivores. Nutrition 2019;66:29–37.

Masoumi-Ardakani Y, Aminizadeh S, Fallah H, Shahouzehi B. L-carnitine different doses affect serum and pancreas tissue Antioxidative defense and histopathology in STZ-induced diabetic rats. Biologia 2020;75(9):1415–23.

Downloads

Published

31-03-2024

How to Cite

1.
Qazi S, Qureshi MA, Qazi AF, Fahim A, Nizamani GS, Qureshi B. EFFECTS OF L-CARNITINE THERAPY ON FAT AND GLUCOSE METABOLISM AMONG MELDONIUM INDUCED CARNITINE DEPLETION IN ALBINO WISTAR RATS: . Pak J Phsyiol [Internet]. 2024 Mar. 31 [cited 2024 May 17];20(1):26-9. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/1610

Issue

Vol. 20 No. 1 (2024): Pakistan Journal of Physiology

Section

Original Article

License

Copyright (c) 2024 Pakistan Journal of Physiology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, cited and quoted with full reference of, and acknowledgement to the PJP.