EFFECT OF SUPPLEMENTATION OF ACACIA HONEY AND PIOGLITAZONE ON INFLAMMATORY MEDIATORS AND OXIDATIVE STRESS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Authors

  • Mahvash Khan RIHS ??, Akhtar Saeed Medical College, Islamabad, Pakistan
  • Umar Ali Khan
  • Bushra Riaz
  • Mehvish Ashfaq
  • Jabran Javaid Department of Physiology, Akhtar Saeed Medical College, Rawalpindi, Pakistan
  • Tallat Naureen

DOI:

https://doi.org/10.69656/pjp.v20i3.1536

Keywords:

Diabetes mellitus, oxidative stress, Honey

Abstract

Background: Diabetes mellitus (DM) has emerged as a major health threat worldwide. There is an increasing advocacy of natural products in the treatment of DM and its complications. Objective of this study was to see the effect of supplementation of Pioglitazone and Acacia honey on inflammatory mediators and oxidative stress in streptozotocin (STZ) induced diabetic rats. Methods: The study was conducted in Al-Nafees Medical College in association with National Institute of Health, Islamabad. There were 150 male Sprague Dawley rats divided into 5 equal groups. Group-I was taken as normal control whereas Group-II was diabetic control. The Groups-II?V were given STZ through intraperitoneal route. Group-III received Acacia honey for 3 weeks. Group-IV was given intra-peritoneal injection of pioglitazone plus Acacia honey, and Group-V was administered pioglitazone through intraperitoneal route for same dose and duration. Serum catalase, hydrogen peroxide, CRP and TNF-? were measured after three weeks. Statistical analysis was done on SPSS-22. The values were presented as Mean±SEM and p?0.05 was considered significant. Results: On day 25 compared to Group-II, serum TNF-? and hydrogen peroxide levels were significantly decreased in Groups-III, IV and V. The mean serum CRP levels were significantly decreased in Group-III and IV while Group-II and III had significantly raised serum catalase levels. Conclusion: Acacia honey along with pioglitazone decreases oxidative stress as predicted by increase in levels of catalase. This combination also reduces inflammation as measured by decreased levels of CRP and TNF-? in STZ induced diabetic rats.

Pak J Physiol 2024;24(3):18?22,  DOI: https://doi.org/10.69656/pjp.v20i3.1536

Downloads

Download data is not yet available.

References

Chaudhury A, Duvoor C, Reddy Dendi VS, Kraleti S, Chada A, Ravilla R, et al. Clinical review of antidiabetic drugs: implications for type 2 diabetes mellitus management. Front Endocrinol 2017;8:6.

Hussain A, Ali I. Diabetes mellitus in Pakistan: A major public health concern. Arch Pharma Pract 2016;7(1):30–2.

Dries SS, Soares BDS, Ziulkoski AL, Verza SG, Linden R, De Andrade FM, et al. Oxidative stress in patients with type 2 diabetes mellitus treated with metformin. Sci Medica 2017;27(2):25857.

Ellulu MS, Samouda H. Clinical and biological risk factors associated with inflammation in patients with type 2 diabetes mellitus. BMC Endocr Disord 2022;22(1):16.

Dumbare M, Kawale L, Nade V, Deshmukh R. Thiazolidine-2, 4-Diones: An update review of antidiabetic agents. Int Res J Pharm 2017;8(12):12–29.

Otero MCB, Bernolo L. Honey as functional food and prospects in natural honey production. In: Egbuna C, Dable Tupas G, (Eds). Functional Foods and Nutraceuticals. Cham: Springer International Publishing; 2020.p. 197–210.

Cr?ciun ME, Parvulescu OC, Donise AC, Dobre T, Stanciu DR. Characterization and classification of Romanian acacia honey based on its physicochemical parameters and chemometrics. Sci Rep 2020;10(1):20690.

Nasrolahi O, Heidari R, Rahmani F, Farokhi F. Effect of natural honey from Ilam and metformin for improving glycemic control in streptozotocin induced diabetic rats. Avicenna J Phytomed 2012;2(4):212–21.

Das S, Mitra K, Mandal M. Sample size calculation: Basic principles. Indian J Anaesth 2016;60:652–56.

Savenije B, Strubbe J, Ritskes-Hoitinga M. Nutrition, feeding and animal welfare. In R. Hubrecht, & J. Kirkwood, (Eds). The UFAW handbook on the care and management of laboratory and other research animals. 8th ed. Wheathampstead, Hertfordshire AL4 8AN, UK: 2010.p. 183–93.

Oribe J, Kakuma T, Haranaka M, Okamoto K, Seike M, Yoshimatsu H. Intraperitoneal administration attenuates thiazolidinedione-induced hepatic steatosis in KKAy mice with increased hepatic peroxisome proliferator-activated receptor (PPAR) ? mRNA expression. Obes Res Clin Pract 2012;6:e175–262.

American Diabetes Association Professional Practice Committee. Prevention or delay of type 2 diabetes and associated comorbidities: Standards of Medical Care in Diabetes ?2022. Diabetes Care 2022;45(Suppl 1):S39–45.

Erejuwa OO, Sulaiman SA, Wahab MS. Honey ?A novel antidiabetic agent. Int J Biol Sci 2012;8(6):913–34.

Singh P, Ishteyaque S, Prajapati R, Yadav KS, Singh R, Kumar A, et al. Assessment of antidiabetic effect of 4-HIL in type 2 diabetic and healthy Sprague Dawley rats. Hum Exp Toxicol 2022;41:9603271211061873.

Tripathi V, Verma J. Different models used to induce Diabetes: A comprehensive review. Int J Pharm Sci 2014;6(6):29–32.

Qujeq D, Revvani T. Catalase (antioxidant enzyme) activity in streptozotocin induced diabetic rats. Int J Diabetes Metab 2007;15:22–4.

Adeshara KA, Bangar N, Diwan AG, Tupe RS. Plasma glycation adducts and various RAGE isoforms are intricately associated with oxidative stress and inflammatory markers in type 2 diabetes patients with vascular complications. Diabetes Metab Synd 2022;16(1):102441.

Erejuwa OO, Gurtu S, Sulaiman SA, Wahab MS, Sirajudeen KN, Salleh MS. Hypoglycemic and antioxidant effects of honey supplementation in streptozotocin induced diabetic rats. Int J Vitam Nutr Res 2010;80:74–82.

Ugusman A, Shahrin SAS, Azizan NH, Pillai SB, Krishnan K, Salamt N, et al. Role of honey in obesity management: A systematic review. Front Nutr 2022;9:924097.

Erejuwa OO, Sulaiman SA, Wahab MS, Salam SK, Salleh MS, Gurtu S. Antioxidant protective effect of Glibenclamide and Metformin in combination with honey in pancreas of streptozotocin induced diabetic rats. Int J Mol Sci 2010;11(5):2056–66.

Varghese A, Asha NS, Celine TM, Prasanna D. Inflammatory markers in type II diabetes mellitus. Pharm Innov J 2015;4:64–6.

Sunarti, Nurliyani, Tyas ASA, Kristian SD, Prasetyastuti. The influence of goat milk and soybean milk kefir on IL-6 and CRP levels in diabetic rats. Rom J Diabetes Nutr Metab Dis 2015;22:261–7.

Asaduzzaman M, Sohanur Rehman M, Munira S, Muedur Rahman M, Hasan M, Siddique MAH, et al. Effects of honey supplementation on hepatic and cardiovascular diseases marker in streptpzotocin induced diabetic rats. J Diabetes Metab 2015;6():592.

Esposito K, Nappo F, Marfella R, Giugliano G, Giugliano F, Ciotola M, et al. Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress. Circulation 2022;106(16):2067–72.

Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, Propolis and Royal Jelly: A comprehensive review of their biological actions and health benefits. Oxid Med Cell Longev 2017;2017:1259510.

Downloads

Published

30-09-2024

How to Cite

1.
Khan M, Khan UA, Riaz B, Ashfaq M, Javaid J, Naureen T. EFFECT OF SUPPLEMENTATION OF ACACIA HONEY AND PIOGLITAZONE ON INFLAMMATORY MEDIATORS AND OXIDATIVE STRESS IN STREPTOZOTOCIN INDUCED DIABETIC RATS. Pak J Phsyiol [Internet]. 2024 Sep. 30 [cited 2024 Oct. 6];20(3):18-22. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/1536