PROPERTIES OF FATIGUE BETWEEN SLOW AND FAST MUSCLES OF MALE AND FEMALE DIABETIC RATS

Authors

  • Mehvish Ashfaq Department of Physiology, HITEC-Institute of Medical Sciences, Taxila, Pakistan
  • Sidra Jabeen Department of Physiology, HITEC-Institute of Medical Sciences, Taxila, Pakistan
  • Sadia Mubarak Department of Physiology, Islamabad Medical and Dental College, Islamabad, Pakistan
  • Amina Rasul Department of Physiology, Watim Medical College, Rawalpindi, Pakistan
  • Sidra Hamid Department of Physiology, Rawalpindi Medical University, Rawalpindi, Pakistan
  • Ambreen Javed Department of Biochemistry, HITEC Institute of Medical Sciences, Taxila, Pakistan

Keywords:

Diabetes mellitus, T2DM, Fast twitch muscle, Muscle Fiber, Fatigue, Slow twitch muscle, Muscle

Abstract

Background: The metabolic disorder of Type 2 Diabetes Mellitus (T2DM) has deleterious effects on skeletal muscle contractile functions. This study aimed to compare parameters of fatigue between slow and fast skeletal muscles of male and female type 2 diabetic Sprague-Dawley rats. Methodology: This was a quasi experimental study conducted at Army Medical College Rawalpindi and National Institute of Health, Islamabad. Forty Sprague Dawley rats in good health were split into groups I (Control) and II (Diabetics) of 20 rats each. Each group had 10 male and 10 female rats. Diabetic groups were given intra-peritoneal streptozotocin (STZ), 35 mg/Kg body weight on 15th day. Control groups were fed a normal diet and diabetic rats were given diet with high fat. On 21st day body weight, blood glucose and triglyceride/high density lipoprotein ratio were estimated to confirm extraction of type 2 diabetes mellitus. Intact Soleus and Extensor Digitorium Longus muscles were removed and fixed in organ bath system having Krebs-Ringer buffer solution and connected to data acquisition unit (iWorx®) to study their fatigability parameters. Independent samples t-test was used for statistical analysis. Results: Slow and fast skeletal muscles in diabetic rats showed reduction in resistance to and recovery from fatigue. Fatigue parameters between male and female diabetic rats revealed no significant differences. Conclusion: Fatigue parameters do not differ between male and female diabetic rats.

Pak J Physiol 2023;19(1):7?10

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Published

31-03-2023

How to Cite

1.
Ashfaq M, Jabeen S, Mubarak S, Rasul A, Hamid S, Javed A. PROPERTIES OF FATIGUE BETWEEN SLOW AND FAST MUSCLES OF MALE AND FEMALE DIABETIC RATS. Pak J Phsyiol [Internet]. 2023 Mar. 31 [cited 2024 Apr. 24];19(1):7-10. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/1514

Issue

Vol. 19 No. 1 (2023): Pakistan Journal of Physiology

Section

Original Article

License

Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, cited and quoted with full reference of, and acknowledgement to the PJP.