PROPERTIES OF FATIGUE BETWEEN SLOW AND FAST MUSCLES OF MALE AND FEMALE DIABETIC RATS
Background: The metabolic disorder of Type 2 Diabetes Mellitus (T2DM) has deleterious effects on skeletal muscle contractile functions. This study aimed to compare parameters of fatigue between slow and fast skeletal muscles of male and female type 2 diabetic Sprague-Dawley rats. Methodology: This was a quasi experimental study conducted at Army Medical College Rawalpindi and National Institute of Health, Islamabad. Forty Sprague Dawley rats in good health were split into groups I (Control) and II (Diabetics) of 20 rats each. Each group had 10 male and 10 female rats. Diabetic groups were given intra-peritoneal streptozotocin (STZ), 35 mg/Kg body weight on 15th day. Control groups were fed a normal diet and diabetic rats were given diet with high fat. On 21st day body weight, blood glucose and triglyceride/high density lipoprotein ratio were estimated to confirm extraction of type 2 diabetes mellitus. Intact Soleus and Extensor Digitorium Longus muscles were removed and fixed in organ bath system having Krebs-Ringer buffer solution and connected to data acquisition unit (iWorx®) to study their fatigability parameters. Independent samples t-test was used for statistical analysis. Results: Slow and fast skeletal muscles in diabetic rats showed reduction in resistance to and recovery from fatigue. Fatigue parameters between male and female diabetic rats revealed no significant differences. Conclusion: Fatigue parameters do not differ between male and female diabetic rats.
Pak J Physiol 2023;19(1):7‒10
Mohammad FH, Nanji K. Risk of Type 2 Diabetes among the Pakistani population: Results of a cross-sectional survey. Cureus 2018;10(8):e3144.
Adnan M, Aasim M. Prevalence of type 2 diabetes mellitus in adult population of Pakistan: a meta-analysis of prospective cross-sectional surveys. Ann Glob Health 2020;86(1):7.
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014;37(Suppl 1):S81–90.
Virgen-Ortiz A, Apolinar-Iribe A, Muñiz J. Gender-effect on the contractile properties of skeletal muscle in streptozotocin-induced diabetic rats. J Musculoskel Neuronal Interact 2018;18(2):255–61.
Pataky MW, Yu CS, Nie Y, Arias EB, Singh M, Mendias CL, et al. Skeletal muscle fiber type-selective effects of acute exercise on insulin-stimulated glucose uptake in insulin resistant, high fat-fed rats. Am J Physiol Endocrinol Metab 2019;16(5):E695–706.
Head SI, Arber MB. An active learning mammalian skeletal muscle lab demonstrating contractile and kinetic properties of fast-and slow-twitch muscle. Adv Physiol Educ 2013;37(4):405–14.
Aleem SB, Hussain MM, Farooq Y. Serum levo-carnitine levels and skeletal muscle functions in type 2 diabetes mellitus in rodents. J Coll Physicians Surg Pak 2013;23(2):132–6.
Duan Y, Li F, Tan B, Yao K, Yin Y. Metabolic control of myofibers: promising therapeutic target for obesity and type 2 diabetes. Obesity Rev 2017;18(6):647–59.
Rasul A, Latif N, Ali S, Ashfaq M, Mubarak S, Rehman I. Taurine supplementation normalizes skeletal muscle functions in type 2 diabetes rats. Pak Armed Forces Med J 2020;29(1):224–9.
Ghelani H, Razmovski‐Naumovski V, Nammi S. Chronic treatment of (R)‐α‐lipoic acid reduces blood glucose and lipid levels in high‐fat diet and low‐dose streptozotocin‐induced metabolic syndrome and type 2 diabetes in Sprague‐Dawley rats. Pharmacol Res Perspect 2017;5(3):e00306
Engel H, Xiong L, Reichenberger MA, Germann G, Roth C, Hirche C. Rodent models of diet-induced type 2 diabetes mellitus: A literature review and selection guide. Diabetes Metab Syndr 2019;13(1):195–200.
Jaishree V, Narsimha S. Swertiamarin and quercetin combination ameliorates hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced type 2 diabetes mellitus in wistar rats. Biomed Pharmacother 2020;130:110561.
Coppey LJ, Shevalye H, Obrosov A, Davidson EP, Yorek MA. Determination of peripheral neuropathy in high‐fat diet fed low‐dose streptozotocin‐treated female C57Bl/6J mice and Sprague-Dawley rats. J Diabetes Investig 2018;9(5):1033–40.
Imam KA, Sarwar M, Wali U, Siddique L, Perveen S. Gender and contractile functions of slow and fast skeletal muscles in streptozotocin induced diabetic Sprague Dawley rats. Romanian J Diabetes Nutr Metab Dis 2012;19(4):417–24.
Dial AG, Monaco CMF, Grafham GK, Patel TP, Tarnopolsky MA, Hawke TJ. Impaired function and altered morphology in the skeletal muscles of adult men and women with type 1 diabetes. J Clin Endocrinol Metab 2021;106(8):2405–22.
Bibi Y, Hussain MM, Naz R. Effect of levocarnitine on endurance capacity in type-2 diabetic rats. J Ayub Med Coll Abbottabad 2013;25(3-4):64–7.
Orlando G, Balducci S, Bazzucchi I, Pugliese G, Sacchetti M. The impact of type 1 diabetes and diabetic polyneuropathy on muscle strength and fatigability. Acta Diabetol 2017;54(6):543–50.
Copyright (c) 2023 Pakistan Journal of Physiology
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, cited and quoted with full reference of, and acknowledgement to the PJP.