PROTECTIVE ROLE OF OLIVE OIL IN THE DOXORUBICIN INDUCED OVARIAN TOXICITY IN FEMALE RATS

Abstract

Background: The pathophysiological effect of chemotherapy on female reproductive system is complex. The current study aimed to determine the protective effect of olive oil extract against doxorubicin induced ovarian toxicity in female rats. Methods: This was an analytical experimental randomized control study carried out in the Department of Anatomy, Peshawar Medical College. Twenty-four female rats weighing 200–250 g were included and were divided into 3 groups, i.e., control group, experimental group-I and experimental group-II. Animals were weighed at the beginning of the experiment and before their sacrifice. The ovaries were obtained after their sacrifice. Five (5) µm thick sections were cut and stained with H & E stain, and Massan Trichome to see the changes the ovarian follicles. Data was entered and analysed on SPSS-20. Independent sample t-test and Chi-square test were used to measure the difference between and within groups, and p?0.05 was considered statistically significant. Results: A significant increase in the mean weight of rats in control group and experimental group-II was observed (p<0.05) while a post-experiment significant decrease in the mean body weight of rats in experimental group-I was seen (p>0.05). There was no significant difference in number of primordial and secondary ovarian follicles within the groups (p>0.05) while a statistical significant difference in number on primary ovarian follicles, atretic follicles and nuclear fragments within the groups (p<0.05). Conclusion: The co-administration of olive oil has shown a protective effects against the doxorubicin induced ovarian toxicity in female rats.

Pak J Physiol 2023;19(3):31–5