PROTECTIVE ROLE OF OLIVE OIL IN THE DOXORUBICIN INDUCED OVARIAN TOXICITY IN FEMALE RATS
DOI:
https://doi.org/10.69656/pjp.v19i3.1507Keywords:
Doxorubicin, Ovarian toxicity, female rats, chemotherapy, Olive oilAbstract
Background: The pathophysiological effect of chemotherapy on female reproductive system is complex. The current study aimed to determine the protective effect of olive oil extract against doxorubicin induced ovarian toxicity in female rats. Methods: This was an analytical experimental randomized control study carried out in the Department of Anatomy, Peshawar Medical College. Twenty-four female rats weighing 200–250 g were included and were divided into 3 groups, i.e., control group, experimental group-I and experimental group-II. Animals were weighed at the beginning of the experiment and before their sacrifice. The ovaries were obtained after their sacrifice. Five (5) µm thick sections were cut and stained with H & E stain, and Massan Trichome to see the changes the ovarian follicles. Data was entered and analysed on SPSS-20. Independent sample t-test and Chi-square test were used to measure the difference between and within groups, and p?0.05 was considered statistically significant. Results: A significant increase in the mean weight of rats in control group and experimental group-II was observed (p<0.05) while a post-experiment significant decrease in the mean body weight of rats in experimental group-I was seen (p>0.05). There was no significant difference in number of primordial and secondary ovarian follicles within the groups (p>0.05) while a statistical significant difference in number on primary ovarian follicles, atretic follicles and nuclear fragments within the groups (p<0.05). Conclusion: The co-administration of olive oil has shown a protective effects against the doxorubicin induced ovarian toxicity in female rats.
Pak J Physiol 2023;19(3):31–5
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References
Kim J, Park EY, Kim O, Schilder JM, Coffey DM, Cho CH, et al. Cell origins of high-grade serous ovarian cancer. Cancers 2018;10(11):433.
Anderson RA, Cameron D, Clatot F, Demeestere I, Lambertini M, Nelson SM, et al. Anti-Müllerian hormone as a marker of ovarian reserve and premature ovarian insufficiency in children and women with cancer: a systematic review. Hum Reprod Update 2022;28(3):417–34.
Kim S, Kim SW, Han SJ, Lee S, Park HT, Song JY, et al. Molecular mechanism and prevention strategy of chemotherapy-and radiotherapy-induced ovarian damage. Int J Mol Sci 2021;22(14):7484.
Bedoschi G, Navarro PA, Oktay K. Chemotherapy-induced damage to ovary: mechanisms and clinical impact. Future Oncol 2016;12(20):2333–44.
Blumenfeld Z. Fertility preservation using GnRH agonists: rationale, possible mechanisms, and explanation of controversy. Clin Med Insights Reprod Health 2019;13:1179558119870163.
Khongorzul P, Ling CJ, Khan FU, Ihsan AU, Zhang J. Antibody–drug conjugates: a comprehensive review. Mol Cancer Res 2020;18(1):3–19.
Kciuk M, Gieleci?ska A, Ko?at D, Ka?uzi?ska ?, Kontek R. Cancer-associated transcription factors in DNA damage response. Biochim Biophys Acta Rev Cancer 2022;1877(4):188757.
Onder GO, Balcioglu E, Baran M, Ceyhan A, Cengiz O, Suna PA, et al. The different doses of radiation therapy-induced damage to the ovarian environment in rats. Int J Radiat Biol 2021;97(3):367–75.
Wu Y, Chen M, Jiang J. Mitochondrial dysfunction in neurodegenerative diseases and drug targets via apoptotic signalling. Mitochondrion 2019;49:35–45.
Visioli F, Davalos A, López de las Hazas MC, Crespo MC, Tomé?Carneiro J. An overview of the pharmacology of olive oil and its active ingredients. Br J Pharmacol 2020;177(6):1316–30.
Swamy AV, Gulliaya S, Thippeswamy A, Koti BC, Manjula DV. Cardioprotective effect of curcumin against doxorubicin-induced myocardial toxicity in albino rats. Indian J Pharmacol 2012;44(1):73–7.
Morgan S, Lopes F, Gourley C, Anderson RA, Spears N. Cisplatin and doxorubicin induce distinct mechanisms of ovarian follicle loss; imatinib provides selective protection only against cisplatin. PloS One 2013;8(7):e70117.
Bar-Joseph H, Ben-Aharon I, Rizel S, Stemmer SM, Tzabari M, Shalgi R. Doxorubicin-induced apoptosis in germinal vesicle (GV) oocytes. Reprod Toxicol 2010;30(4):566–72.
Zhang T, He WH, Feng LL, Huang HG. Effect of doxorubicin-induced ovarian toxicity on mouse ovarian granulosa cells. Regul Toxicol Pharmacol 2017;86:1–10.
Brayboy LM, Oulhen N, Long S, Voigt N, Raker C, Wessel GM. Multidrug resistance transporter-1 and breast cancer resistance protein protect against ovarian toxicity, and are essential in ovarian physiology. Reprod Toxicol 2017;69:121–31.
Al-Kawlani B, Murrieta-Coxca JM, Chaiwangyen W, Fröhlich K, Fritzsche A, Winkler S, et al. Doxorubicin induces cytotoxicity and miR-132 expression in granulosa cells. Reprod Toxicol 2020;96:95–101.
Renu K, Pureti LP, Vellingiri B, Valsala Gopalakrishnan A. Toxic effects and molecular mechanism of doxorubicin on different organs–an update. Toxin Rev 2022;41(2):650–74.
Samare-Najaf M, Zal F, Safari S. Primary and secondary markers of doxorubicin-induced female infertility and the alleviative properties of quercetin and vitamin E in a rat model. Reprod Toxicol 2020;96:316–26.
Granados-Principal S, Quiles JL, Ramirez-Tortosa CL, Sanchez-Rovira P, Ramirez-Tortosa M. New advances in molecular mechanisms and the prevention of adriamycin toxicity by antioxidant nutrients. Food Chem Toxicol 2010;48(6):1425–38.
Jab?o?ska-Trypu? A, Kr?towski R, Kalinowska M, ?widerski G, Cechowska-Pasko M, Lewandowski W. Possible mechanisms of the prevention of doxorubicin toxicity by cichoric acid—antioxidant nutrient. Nutrients 2018;10(1):44.
Tocchetti CG, Cadeddu C, Di Lisi D, Femmino S, Madonna R, Mele D, et al. From molecular mechanisms to clinical management of antineoplastic drug-induced cardiovascular toxicity: a translational overview. Antioxid Redox Signal 2019;30(18):2110–53.
Roti Roti EC, Salih SM. Dexrazoxane ameliorates doxorubicin-induced injury in mouse ovarian cells. Biol Reprod 2012;86(3):96.
Mohajeri M, Sahebkar A. Protective effects of curcumin against doxorubicin-induced toxicity and resistance: A review. Crit Rev Oncol Hematol 2018;122:30–51.
Nishi K, Gunasekaran VP, Arunachalam J, Ganeshan M. Doxorubicin-induced female reproductive toxicity: an assessment of ovarian follicular apoptosis, cyclicity and reproductive tissue histology in Wistar rats. Drug Chem Toxicol 2018;41(1):72–81.
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