EFFECT OF TAURINE ON BIOMARKER OF OXIDATIVE STRESS IN SERUM OF HIGH FAT DIET-LOW DOSE STREPTOZOTOCIN INJECTED MODEL OF TYPE 2 DIABETIC RATS

Abstract

Background: Oxidative stress plays a major role in the development of insulin resistance and pancreatic beta cell dysfunction. Taurine and beta-alanine have been documented to act as antioxidants. The aim of this study was to compare the antioxidant effect of taurine and beta-alanine in type 2 diabetic rats. Methods: This Laboratory based experimental study was conducted in Department of Physiology at Army Medical College, Rawalpindi, in collaboration with National Institute of Health, Islamabad, from July to Sep 2018. Ninety male Sprague-Dawley rats were randomly divided into three groups; diabetic control (DC), diabetic beta-alanine (DBA), and diabetic taurine group (DTau). All ninety rats were fed with taurine free-high fat diet for a period of four weeks, and administered low-dose streptozocin on the 14^th day for the induction of type 2 diabetes mellitus. Also, DC rats were supplemented with 0.02% (w/v) taurine, DBA rats with 3% (w/v) beta-alanine, and DTau rats with 3% (w/v) taurine in their respective drinking water, for a period of four weeks. At day 21, plasma glucose levels and insulin resistance were measured to confirm development of type 2 diabetes mellitus in the three groups. At the end of four weeks, terminal intracardiac sampling was done to measure 8-isoprostane levels. Results: 8-isoprostane levels were significantly increased in diabetic group. Taurine supplementation ameliorated these effects as compared to beta-alanine. Conclusion: Taurine reduces oxidative stress in type 2 diabetes mellitus.

Pak J Physiol 2022;18(3):11?4