PROTECTIVE EFFECTS OF SILYMARIN AND CHOLCALCIFEROL ON ISONIAZID INDUCED HEPATOTOXICITY IN MICE

Authors

  • Rabia Iftikhar Malik Shifa College of Medicine, Islamabad, Pakistan
  • Akbar Waheed Islamic International Medical College, Riphah University, Islamabad, Pakistan
  • Salma Salim Mohi-ud-Din Islamic Medical College , Mirpur, Azad Jammu & Kashmir
  • Fatima Muhammadi Islamic International Medical College, Riphah University, Islamabad, Pakistan
  • Nimra Ijaz Fazaia Medical College, Air University, Islamabad, Pakistan
  • Uzma Naeem Islamic International Medical College, Riphah University, Islamabad, Pakistan

Keywords:

Silymarin, Hepatotoxicity, Isoniazid, Cholecalciferol

Abstract

Background: Hepatotoxicity is the gravest concern associated with use of anti-tuberculous drugs. The objective of this study was to evaluate the individual and combined hepatoprotective effect of silymarin and cholecalciferol in isoniazid induced hepatotoxicity. Methods: This animal experimental study was conducted at the Department of Pharmacology & Therapeutics, and Multidisciplinary Research Laboratory, Islamic International Medical College, in collaboration with National Institute of Health, Islamabad, Pakistan. Fifty adult Balb-C mice were included in this study. They were distributed into 5 groups. Each group contained 10 mice. Group 1 was normal control, Group 2 disease control, and Group 3, 4, and 5 were experimental groups. Except Group 1, all other groups were given isoniazid (150 mg/Kg) and only Group 2 was not fed with any drugs. Group 3 received silymarin (50 mg/Kg dissolved in physiological saline) through intragastric gavage for 28 days. Group 4 was given Vitamin D (1,000 IU/Kg) for 28 days. Group 5 was given isoniazid (150 mg/Kg) along with silymarin and Vitamin D for 28 days. Serum ALT and bilirubin levels were estimated on day 0, 14, and 28. Results: As compared to Group 2, Group 3 to Group 5 showed a lower rise in serum ALT and bilirubin (p<0.001). Group 4 and 5 showed significantly reduced biochemical markers (ALT and bilirubin) (p=0.001). Conclusion: Silymarin and cholecalciferol effectively and synergistically ameliorate hepatotoxicity induced by isoniazid. Silymarin offers better hepatoprotection than cholecalciferol in isoniazid induced hepatotoxicity.

Pak J Physiol 2024;20(1):37-40

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Published

31-03-2024

How to Cite

1.
Malik RI, Waheed A, Salim S, Muhammadi F, Ijaz N, Naeem U. PROTECTIVE EFFECTS OF SILYMARIN AND CHOLCALCIFEROL ON ISONIAZID INDUCED HEPATOTOXICITY IN MICE. Pak J Phsyiol [Internet]. 2024 Mar. 31 [cited 2024 Jun. 16];20(1):37-40. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/1450

Issue

Vol. 20 No. 1 (2024): Pakistan Journal of Physiology

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Original Article

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Copyright (c) 2024 Rabia Iftikhar Malik, Akbar Waheed, Salma Salim, Fatima Muhammadi, Nimra Ijaz, Uzma Naeem

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