Bitter Coffee for Sweet Diabetes: A Randomized Controlled Trial For Treatment Of Type 2 Diabetes With Black Coffee In Balb c Albino Mice
Background: Diabetes Mellitus is a chronic metabolic ailment which slowly but surely harms the human body if left untreated. The objective of this study was to determine the effect of black coffee on HbA1C, fasting and postprandial blood sugar levels in mice model of type 2 diabetes mellitus. Methods: This was an experimental, randomized control study performed at the Pharmacology Laboratory, Multidisciplinary Research Laboratory at Islamic International Medical College and National Institute of Health (NIH) Islamabad Pakistan. The study comprised a total of 30 male Balb/c albino mice and diabetes was induced in experimental group (n=20) by using low dose streptozotocin (40 mg/Kg). After confirmation, diabetic mice were further divided into two groups of 10 each. Group 2 was diabetic control and Group 3 was treated with black coffee for 45 days. Blood samples were taken from lateral tail vein for fasting and post prandial blood sugar levels and by intracardiac puncture for HbA1c. Statistical analysis was done on SPSS-21. Comparisons between the groups were analyzed using one way ANOVA (post hoc tuckey test), and p<0.05 was considered significant. Results: Black coffee treated mice (Group 3) had significantly decreased serum HbA1C levels (6.02±0.29) fasting (116.8±4.92) and postprandial (173.6±18.3) blood glucose levels in comparison with those found in diabetic control mice (Group 2). Conclusion: Black coffee significantly decreases serum HbA1C, fasting and postprandial blood glucose levels in diabetic mice.
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Olokoba AB, Obateru OA, Olokoba LB. Type 2 diabetes mellitus: a review of current trends. Oman medical journal. 2012;27(4):269.
Chen L, Magliano DJ, Zimmet PZ. The worldwide epidemiology of type 2 diabetes mellitus—present and future perspectives. Nature reviews endocrinology. 2012;8(4):228.
Ighodaro OM. Molecular pathways associated with oxidative stress in diabetes mellitus. Biomedicine & Pharmacotherapy. 2018;108:656-62.
Zafar J, Bhatti F, Akhtar N, Rasheed U, Bashir R, Humayun S, et al. Prevalence and risk factors for diabetes mellitus in a selected urban population of a city in Punjab. JPMA-Journal of the Pakistan Medical Association. 2011;61(1):40.
Meo SA, Zia I, Bukhari IA, Arain SA. Type 2 diabetes mellitus in Pakistan: Current prevalence and future forecast. JPMA The Journal of the Pakistan Medical Association. 2016;66(12):1637-42.
Abbasi A, Juszczyk D, van Jaarsveld CHM, Gulliford MC. Body Mass Index and Incident Type 1 and Type 2 Diabetes in Children and Young Adults: A Retrospective Cohort Study. Journal of the Endocrine Society. 2017;1(5):524-37.
Atlas D. International diabetes federation. IDF Diabetes Atlas, 7th edn Brussels, Belgium: International Diabetes Federation. 2015.
Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nature Reviews Endocrinology. 2018;14(2):88-98.
Basit A, Fawwad A, Qureshi H, Shera A. Prevalence of diabetes, pre-diabetes and associated risk factors: second National Diabetes Survey of Pakistan (NDSP), 2016–2017. BMJ open. 2018;8(8):e020961.
Cho N, Shaw J, Karuranga S, Huang Y, da Rocha Fernandes J, Ohlrogge A, et al. IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes research and clinical practice. 2018;138:271-81.
Association AD. Standards of medical care in diabetes—2010. Diabetes care. 2010;33(Supplement 1):S11-S61.
Kleinert M, Clemmensen C, Hofmann SM, Moore MC, Renner S, Woods SC, et al. Animal models of obesity and diabetes mellitus. Nature Reviews Endocrinology. 2018;14(3):140-62.
Camandola S, Plick N, Mattson MP. Impact of coffee and cacao purine metabolites on neuroplasticity and neurodegenerative disease. Neurochemical research. 2019;44(1):214-27.
Mirmiran P, Carlström M, Bahadoran Z, Azizi F. Long-term effects of coffee and caffeine intake on the risk of pre-diabetes and type 2 diabetes: findings from a population with low coffee consumption. Nutrition, Metabolism and Cardiovascular Diseases. 2018;28(12):1261-6.
Casal S, Rebelo I. Coffee: a dietary intervention on type 2 diabetes? Current medicinal chemistry. 2017;24(4):376-83.
Arulmozhi DK, Kurian R, Bodhankar SL, Veeranjaneyulu A. Metabolic effects of various antidiabetic and hypolipidaemic agents on a high‐fat diet and multiple low‐dose streptozocin (MLDS) mouse model of diabetes. Journal of Pharmacy and Pharmacology. 2008;60(9):1167-73.
Jin S, Chang C, Zhang L, Liu Y, Huang X, Chen Z. Chlorogenic acid improves late diabetes through adiponectin receptor signaling pathways in db/db mice. PLoS One. 2015;10(4).
Kobayashi M, Kurata T, Hamana Y, Hiramitsu M, Inoue T, Murai A, et al. Coffee ingestion suppresses hyperglycemia in streptozotocin-induced diabetic mice. Journal of nutritional science and vitaminology. 2017;63(3):200-7.
Cao H, Ou J, Chen L, Zhang Y, Szkudelski T, Delmas D, et al. Dietary polyphenols and type 2 diabetes: Human Study and Clinical Trial. Critical Reviews in Food Science and Nutrition. 2019;59(20):3371-9.
Riany H. Effects of Coffee Consumption In Improving Hyperglicemia In Diabetes-Induced Mice. International Journal of Ecophysiology. 2019;1(1):72-80.
Martina SJ, Govindan PA, Wahyuni AS. The Difference in Effect of Arabica Coffee Gayo Beans and Leaf (Coffea Arabica Gayo) Extract on Decreasing Blood Sugar Levels in Healthy Mice. Open access Macedonian journal of medical sciences. 2019;7(20):3363.
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