• Hamid Hassan Nishtar Medical University, Multan
  • Muhammad Abdullah Nishtar Medical University, Multan
  • Muhammad Ahmad Nishtar Medical University, Multan
  • Muhammad Muzammil Nishtar Medical Univerity, multan. Pakistan
  • Zunairah Humayun Multan Medical & Dental College, Multan
  • Mariyam Khan Nishtar Medical University, Multan
  • Mehreen Zaidi Nishtar Medical University, Multan
  • Saadat Ali Khan Multan Medical & Denatl College, Multan
Keywords: obesity, testosterone, aging men, waist hip ratio, waist circumference, body mass index


Background: Aging men experience gradual decline in serum testosterone levels (andropause) which is accentuated if aging is coupled with obesity. This study aimed to note the age and obesity related testosterone decline in men. Methods: It was a cross-sectional study comprising of 80 healthy male subjects categorized into younger (20–40 years) and elder (41–60 years) groups which were equally divided into non-obese and obese subgroups. Serum testosterone levels were measured using ELISA. Results: Serum testosterone levels (ng/dl) of young non-obese subjects (Group A) were significantly higher [680 (575.0–778.5)] as compared to their elder (Group B) [286.0 (263.5–370.0)] counterparts (p=0.000), and so was true for comparison between (Group C) younger [412.5 (338.0–542.5)] and (Group D) elder obese subjects [258.0 (220.0–287.5)] (p=0.000). Serum testosterone levels of obese elder subjects (group D), though lower than their age and ethnicity matched non-obese (Group B) counterparts, were not statistically significant (p=0.114). Moreover, serum testosterone levels of non-obese (Group A+B) subjects were negatively correlated to Waist Circumference (WC) and Waist Hip Ratio (WHR) [(rho= -0.374, p=0.018) and (rho= -0.355, p=0.025) respectively] while within obese subjects (Group C+D) serum testosterone levels were negatively correlated to waist circumference only [(rho= -0.643, p=0.000)]. Conclusion: Circumferential obesity coupled with aging results in a steeper decline in serum testosterone levels which can puts obese aging men at high risk of systemic disorders.


Corriger A, Duclos M, Corcuff JB, Lambert C, Marceau G, Sapin V, et al. Hormonal status and cognitivo-emotional profile in real-life patients with neuropathic pain: A case control study. Pain Pract 2019;19(7):703–14.

Samipoor F, Pakseresht S, Rezasoltani P, Kazemnajad Leili E. Awareness and experience of andropause symptoms in men referring to health centers: a cross-sectional study in Iran. Aging Male 2017;20(3):153–60.

Veldhuis J, Yang R, Roelfsema F, Takahashi P. Proinflammatory cytokine infusion attenuates LH’s feedforward on testosterone secretion: modulation by age. J Clin Endocrinol Metab 2016;101(2):539–49.

Ayaz O, Howlett SE. Testosterone modulates cardiac contraction and calcium homeostasis: cellular and molecular mechanisms. Biol Sex Differ 2015;6:9.

Amjad S, Baig M, Zahid N, Tariq S, Rehman R. Association between leptin, obesity, hormonal interplay and male infertility. Andrologia 2019;51(1):e13147.

Amin F, Fatima SS, Islam N, Gilani AH. Prevalence of obesity and overweight, its clinical markers and associated factors in a high risk South-Asian population. BMC Obes 2015;2:16.

Siddiqui M, Ayub H, Hameed R, Nadeem MI, Mohammad TA, Simbak N, et al. Obesity in Pakistan: Current and future perceptions. Curr Trends Biomed Eng Biosci 2018;17(2):555958.

Gadekar T, Dudeja P, Basu I, Vashisht S, Mukherji S. Correlation of visceral body fat with waist-hip ratio, waist circumference and body mass index in healthy adults: A cross sectional study. Med J Armed Forces India 2020;76(1):41–6.

Morgan A, Mooney K, Mc Auley M. Obesity and the dysregulation of fatty acid metabolism: implications for healthy aging. Expert Rev Endocrinol Metab 2016;11(6):501–10.

Rodrigues KF, Pietrani NT, Bosco AA, Campos FMF, Sandrim VC, Gomes KB. IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals. Arch Endocrinol Metab 2017;61(5):438–46.

Landecho MF, Tuero C, Valentí V, Bilbao I, de la Higuera M, Frühbeck G. Relevance of leptin and other adipokines in obesity-associated cardiovascular risk. Nutrients 2019;11(11):2664.

Zakharova I, Klimov L, Kuryaninova V, Nikitina I, Malyavskaya S, Dolbnya S, et al. Vitamin D insufficiency in overweight and obese children and adolescents. Front Endocrinol (Lausanne) 2019;10:103.

Harman SM, Metter EJ, Tobin JD, Pearson J, Blackman MR. Longitudinal effects of aging on serum total and free testosterone levels in healthy men. J Clin Endocrinol Metab 2001;86(2):724–31.

Cohen J, Nassau DE, Patel P, Ramasamy R. Low testosterone in adolescents & young adults. Front Endocrinol (Lausanne) 2019;10:916.

Fui MN, Dupuis P, Grossmann M. Lowered testosterone in male obesity: mechanisms, morbidity and management. Asian J Androl 2014;16(2):223–31.

Lee HK, Lee JK, Cho B. The role of androgen in the adipose tissue of males. World J Mens Health 2013;31(2):136–40.

Bekaert M, Van Nieuwenhove Y, Calders P, Cuvelier CA, Batens AH, Kaufman JM, et al. Determinants of testosterone levels in human male obesity. Endocrine 2015;50(1):202–11.

Cooper LA, Page ST, Amory JK, Anawalt BD, Matsumoto AM. The association of obesity with sex hormone‐binding globulin is stronger than the association with aging –implications for the interpretation of total testosterone measurements. Clin Endocrinol (Oxf) 2015;83(6):828–33.

Van de Velde F, Reyns T, Toye K, Fiers T, Kaufman JM, T'Sjoen G, et al. The effects of age and obesity on postprandial dynamics of serum testosterone levels in men. Clin Endocrinol (Oxf) 2020;92(3):214–21.

Sartorius G, Spasevska S, Idan A, Turner L, Forbes E, Zamojska A, et al. Serum testosterone, dihydrotestosterone and estradiol concentrations in older men self‐reporting very good health: The healthy man study. Clin Endocrinol (Oxf) 2012;77(5):755–63.

Zeller T, Appelbaum S, Kuulasmaa K, Palosaari T, Blankenberg S, Jousilahti P, et al. Predictive value of low testosterone concentrations regarding coronary heart disease and mortality in men and women –evidence from the FINRISK 97 study. J Intern Med 2019;286(3):317–25.

DeMorrow S. Role of the Hypothalamic-Pituitary-Adrenal Axis in Health and Disease. Int J Mol Sci 2018;19(4):986.

Kurniawan LB, Adnan E, Windarwati, Mulyono B. Insulin resistance and testosterone level in Indonesian young adult males. Rom J Intern Med 2020;58(2):93‒8.

Shamim MO, Khan FM, Arshad R. Association between serum total testosterone and Body Mass Index in middle aged healthy men. Pak J Med Sci 2015;31(2):355–9.

Yassin AA, Nettleship JE, Salman M, Almehmadi Y. Waist circumference is superior to weight and BMI in predicting sexual symptoms, voiding symptoms and psychosomatic symptoms in men with hypogonadism and erectile dysfunction. Andrologia 2017;49(4):e12634.

Zitzmann M, Nieschlag E. Testosterone levels in healthy men and the relation to behavioural and physical characteristics: facts and constructs. Eur J Endocrinol 2001;144(3):183–97.

Lima N, Cavaliere H, Knobel M, Halpern A, Medeiros-Neto G. Decreased androgen levels in massively obese men may be associated with impaired function of the gonadostat. Int J Obes Relat Metab Disord 2000;24(11):1433–7.


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How to Cite
Hassan H, Abdullah M, Ahmad M, Muzammil M, Humayun Z, Khan M, Zaidi M, Khan S. COMBINED EFFECTS OF AGING AND OBESITY ON SERUM TESTOSTERONE LEVELS OF OTHERWISE HEALTHY MALES OF SOUTH PUNJAB. PJP [Internet]. 30Sep.2021 [cited 18Jan.2022];17(3):27-1. Available from: