Role of antioxidant in chemotherapy related infertility

  • Aisha Abdul Haq Anatomy Department, DUHS
  • Maria Khan
  • Shamaila Khalid DUHS
  • Soofia Nigar DUHS
  • Sarwat Jabeen
  • Hina Abbas
Keywords: Doxorubicin, Vitamin C, oxidative damage, sperms, mice, Infertility, Epididymism

Abstract

Background: Chemotherapeutic agent Doxorubicin (DOX), an anthracycline induced gonadotoxic damage has concerned physicians for decades. DOX induces damage on healthy tissues through the formation of free radicals. Vitamin C, a water-soluble non-enzymatic antioxidant was used to study its protective role on male gonads damaged by DOX. Methods: In this experimental study 30 male mice were divided into 3 groups of 10 mice each, A, B, and C. Group A was given normal saline 1 ml intra-peritoneal (IP). Group B mice were given DOX alone (0.003 mg in 0.03 ml/gm body weight IP for 3 doses on 6th, 8th and 10th day of study). In Group C, DOX (0.003 mg in 0.03 ml/gm body weight IP for 3 doses on 6th, 8th and 10th day of study) was given and Vitamin C (0.5 mg in 0.01 ml/gm body weight per orum daily was added to medication. On completion of experiment, epididymides dissected along with testes from sacrificed animals and were placed in Petri dish containing 5 ml saline and left for a few minutes for spermatozoa to float. Neubauer chamber was used for sperm count. Results: The DOX administration significantly decreased (p<0.001) number and motility of sperms as compared to controls. However co-administration of Vitamin C with DOX significantly reduced (p<0.001) the seminiferous epithelial damage. Sperm parameters seemed to be improved as compared to DOX only and control group respectively. Conclusion: Vitamin C has protective role against DOX induced testicular damage.

Pak J Physiol 2020;16(1):14–6

Author Biographies

Aisha Abdul Haq, Anatomy Department, DUHS

MBBS, MPhil Anatomy

Associate Professor

Anatomy Department

Dow Medical College

Maria Khan

Assistant Professor

Department of Anatomy

DUHS Karachi

Cell#03333826481

Shamaila Khalid, DUHS

MBBS, MPhil pharmacology

Associate Professor

Pharmacology Department

Dow Medical College,

DUHS, Karachi

Soofia Nigar, DUHS

MBBS, MPhil Anatomy

Associate Professor

Anatomy Department

Dow Medical College

DUHS, Karachi

Cell # 03332204437

Sarwat Jabeen

Associate Professor

Anatomy Department

Dow Medical College

DUHS Karachi

Cell # 03332070879

Hina Abbas

Assistant professor

Department of pathology

Dow medical college

DUHS Karachi

CELL# 03323779503

Downloads

Download data is not yet available.
Published
2020-03-31
How to Cite
1.
Haq A, Khan M, Khalid S, Nigar S, Jabeen S, Abbas H. Role of antioxidant in chemotherapy related infertility. PJP [Internet]. 31Mar.2020 [cited 21Sep.2020];16(1):14-6. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/1198
Section
Original Article