EFFECT OF ALLIUM SATIVUM ON EXPERIMENTALLY INDUCED HYPERLIPIDEMIA IN GUINEA PIGS

  • Prema Ram Choudhary Department of Physiology
  • Jaidev Singh Shekhawat Anatomy, CU Shah Medical College, Surendranagar, Gujarat
  • Megh Shyam Sharma Department of Physiology, RNT Medical College, Udaipur, Rajasthan
  • Jyotsna Dashora Department of Physiology, MMIMSR, Mullana Ambala, Haryana, India
Keywords: Allium sativum, alcoholic extract, aqueous extract, lipid profile, hyperlipidemia

Abstract

Background: Garlic (Allium sativum) is naturally occurring sulphur containing dietary agent belongs to family liliaceace. Members of this family like garlic and onions are found to have beneficial effect on atherosclerosis and ischemic heart diseases in experimental animals and humans. The objective of this study was to find out the effect of Allium sativum on experimentally induced hyperlipidemia in guinea pigs. Methods: Twenty-five guinea pigs were fed cholesterol (0.5 g/Kg body weight/day) for an initial period of 4 weeks. Cholesterol was then discontinued and the animals were divided into 3 groups. Group-I (control, n=7) was now fed normal diet with 1 ml normal saline. Group-II (n=9) was given 1 ml of aqueous extract with normal diet, and Group-III (n=9) was fed normal diet with 1 ml of alcoholic extract of garlic daily for 4 weeks. The garlic contents of both extracts were 2 g/ml. Fasting blood samples were collected at the end of 4 weeks after induced hyperlipidemia and finally at the end of the study (i.e., 8 weeks) for estimation of total serum cholesterol, serum triglyceride, HDLc, LDLc, VLDLc and atherogenic index was calculated in all 3 groups. Results: The aqueous and alcoholic extracts of garlic showed a significant hypolipidemic activity as they reduced significantly serum cholesterol, serum triglyceride, LDLc, VLDLc and atheriogenic index in hyperlipidemic guinea pigs (p<0.001) as compared to control group. The significant rise in HDLc was observed in group II but not in group I and III animals. On comparison between the two extracts, aqueous extract of garlic was found to be more potent hypolipidemic agent than to the alcoholic extract. Conclusion: Both extracts have hypolipidemic activity but aqueous extract of Allium sativum is more potent than alcoholic extract.

Pak J Physiol 2013;9(2):38–40

References

1. Banerjee SK, Maulik SK. Effect of garlic on cardiovascular disorders: a review. Nutr J 2002;1(1):1–4.
2. Bakhshi R, Chughtal MI. Influence of garlic on serum cholesterol, serum triglycerides, serum total lipids and serum glucose in human subject. Nahrung 1984;28(2):159–63.
3. Lau BHS, Lam F, Wang-Cheng R. Effect of an odor modified garlic preparation on blood lipid. Nut Res 1987;7:139–49.
4. Qureshi AA, Din ZZ, Abuirmeileh N. Suppression of avain hepatic lipid metabolism by solvent extract of garlic: impact on serum lipid. J Nut 1983;113:1746–55.
5. Bordia A. Effect of garlic on blood lipids in patients with coronary heart disease. Am J Clin Nutr 1981;34:2100–3.
6. Jain RC. Onion and garlic in experimental induced atherosclerosis. Ind J Med Res 1976;76:1508–15.
7. Ziaei S, Hantoshzudeh S, Rexasoltani P, Lamyian. The effect of garlic tablets on plasma lipids and platelets in nulliparous pregnant at high risk of pre-eclampsia. Eur J Obstet Gynecol Reprod Bio 2001;99:201–6.
8. Superko HR, Krauss RM. Cholesterol reduction in cardiovascular disease: clinical benefits and possible mechanism. New Engl J Med 2000;322:512–21.
9. Berthold HK, Sudhop T, Von Bergmann K. Effect of a garlic oil preparation on serum lipoproteins and cholesterol metabolism: a randomized control trial. JAMA 1998;279:1900–2.
10. Allain CC, Poon LS, Chan CS, Richmond W, Fu PC Enzymatic determination of total serum cholesterol. Clin Chem 1974;20(4):470–5.
11. Fossati P, Principle L. Serum triglycerides determined calorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 1982;28(10):2077–80.
12. Sainani GS, Desai DB, Gorhe NH. Effect of dietary garlic and onion on lipid profile of Jain community. Ind J Med Res 1979;69:776–80.
13. Bordia A, Bansal HC, Arora SK. Effect of essential oil of garlic on serum cholesterol, plasma fibrinogen, whole blood coagulation time and fibrinolytic activity in alimentary lipemia. J Assoc Phys Ind 1974;22:267–70.
14. Bordia A, Verma SK, Khabia BL. The effect principle of garlic and onion on blood lipids and experimental atherosclerosis in rabbits and their comparison with clofibrate. J Assoc Phy Ind 1977;25:509–16.
15. Schulz V, Hansel R, Tyler VE, Blumenthal M, (Eds). Rational Phytotherapy: A Physician’s Guide. 5th ed. Berlin: Springer-Verlag; 2004; p.128–38.
16. Yen YY, Liu L. Cholesterol lowering effect of garlic extracts and organo-sulpur compounds: Human and animal studies. J Nutr 2001;131(3s):989s–93s.

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Published
2013-12-31
How to Cite
1.
Choudhary P, Shekhawat J, Sharma M, Dashora J. EFFECT OF ALLIUM SATIVUM ON EXPERIMENTALLY INDUCED HYPERLIPIDEMIA IN GUINEA PIGS. PJP [Internet]. 31Dec.2013 [cited 9Apr.2020];9(2):38-0. Available from: http://pjp.pps.org.pk/index.php/PJP/article/view/389