CO-EXISTENCE OF CIPROFLOXACIN RESISTANCE IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS FROM CLINICAL SAMPLES

Authors

  • Atif A. Patoli Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
  • Bushra B. Patoli Institute of Microbiology, University of Sindh, Jamshoro, Pakistan
  • Zulifqar A. Laghari Department of Physiology, University of Sindh, Jamshoro
  • Taj M. Maachi Department of Medicine, Agha Khan University, Karachi

Keywords:

Staphylococcus aureus, Clinical Specimens, Ciprofloxacin, Methicillin

Abstract

Background: Ciprofloxacin is a broad-spectrum, fluorinated quinolone antibiotic. It has been successfully prescribed against a variety of bacteria. Though its use was limited to a last resort therapy against complicated infections, however widespread usage has led to the emergence of ciprofloxacin resistance. The current study was planned to evaluate the existence of ciprofloxacin resistance in methicillin resistant and sensitive Staphylococcus aureus from clinical samples. Methods: The clinical S. aureus isolates from wound, blood, urine and nose, were obtained from various labs of Hyderabad over nine months. The methicillin resistant strains were identified by Kirbey baur disc diffusion test using oxacillin (1 µg) discs. The response of Methicillin Resistant S. aureus and Methicillin Sensitive S. aureus strains against 2nd generation flouroquinolone, i.e., ciprofloxacin was demonstrated. Results: A total of 150 S. aureus isolates from various clinical specimens were included in this study. About 14.6 % (n=22) showed resistance against ciprofloxacin while 30% (n=48) were identified as MRSA. About 25% of the Methicillin Resistant S. aureus (MRSA) isolates displayed the co-resistance against ciprofloxacin. Among various specimens the maximum co-resistance was seen in case of blood isolates (37.5%) followed by urine isolates (33.3%). Comparatively, ciprofloxacin resistance was found to be lower in Methicillin Sensitive S. aureus (MSSA) strains (9.1%). Odds Ratio [OR] was calculated to be 0.30 [95% CI=0.12–0.77]. Statistically significant differences (p˂0.05) for ciprofloxacin resistance were seen between MRSA and MSSA. Conclusion: The results suggest a statistically significant increase of ciprofloxacin resistance in Methicillin Resistant S. aureus as compared to Methicillin Sensitive S. aureus in clinical samples.

Pak J Pysiol 2018;14(2)24–7

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Pak J Physiol 2018;14(2)

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Published

30-06-2018

How to Cite

1.
Patoli AA, Patoli BB, Laghari ZA, Maachi TM. CO-EXISTENCE OF CIPROFLOXACIN RESISTANCE IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS FROM CLINICAL SAMPLES. Pak J Phsyiol [Internet]. 2018 Jun. 30 [cited 2024 Apr. 24];14(2):24-7. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/224

Issue

Vol. 14 No. 2 (2018): Pakistan Journal of Physiology

Section

Original Article

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Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, cited and quoted with full reference of, and acknowledgement to the PJP.