EFFECTS OF ASCORBIC ACID AND ALPHA TOCOPHEROL SUPPLEMENTATION ON BASAL TESTOSTERONE CORTISOL RATIO IN MALE SPRAGUE DAWLEY RATS

  • Ghulam Mustafa Lodhi Department of Physiology, Al-Nafees Medical College, Farash Town, Lehtrar Road, Islamabad, Pakistan.
  • Waqas Hameed Pak International Medical College, Peshawar
  • Tausif Ahmed Department of Biochemistry, Army Medical College, Rawalpindi,
  • Rabia Latif Department of Physiology, College of Medicine, University of Dammam, Saudi Arabia
  • Muhammad Aslam Shifa College of Medicine, Islamabad, Pakistan
Keywords: Testosterone cortisol ratio, Ascorbic acid, Alpha tocopherol, Malondialdehyde

Abstract

Background: Basal testosterone cortisol ratio is considered very important to maintain homeostasis. Increase in this ratio has various beneficial effects on body. In this study we determined the effects of ascorbic acid and alpha tocopherol supplementation on basal testosterone cortisol ratio in male Sprague Dawley rats. Methods: It was quasi experimental study carried out in department of Physiology, Army Medical College Rawalpindi in collaboration with National Institute of Health, Islamabad during October 2006 to September 2007. Forty male Sprague Dawley rats were divided into four groups with ten rats in each group and above mentioned antioxidants supplementation were given along with standard diet for one month. After this, blood samples were taken and analyzed for serum testosterone and cortisol by ELISA and malondialdehyde levels colorimetrically. Data were analysed on SPSS version 13 and p<0.05 was considered significant. Results: There was no significant rise in testosterone cortisol ratio in rats supplemented with single antioxidant; however rats supplemented with combination of ascorbic acid and alpha tocopherol revealed significant rise in testosterone cortisol ratio with a fall in malondialdehyde levels. Conclusions: Synergistic effects of ascorbic acid and alpha tocopherol resulted in a decline in reactive oxygen species induced lipid peroxidation and rise of testosterone cortisol ratio.

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Published
2018-05-28
How to Cite
1.
Lodhi G, Hameed W, Ahmed T, Latif R, Aslam M. EFFECTS OF ASCORBIC ACID AND ALPHA TOCOPHEROL SUPPLEMENTATION ON BASAL TESTOSTERONE CORTISOL RATIO IN MALE SPRAGUE DAWLEY RATS. PJP [Internet]. 28May2018 [cited 8Mar.2021];7(2):7-1. Available from: http://pjp.pps.org.pk/index.php/PJP/article/view/805