EFFECT OF GHRELIN ANTAGONIST ON FOOD AND WATER INTAKE IN OBESE AND TYPE 2 DIABETIC MICE

Authors

  • Lubna Siddique Department of Physiology, Margalla Institute of Health Sciences, Rawalpindi
  • Madiha Imran Foundation University Medical College, Islamabad

Keywords:

Ghrelin, Ghrelin antagonist, Obesity, Diabetes, T2DM

Abstract

Background: Ghrelin is the most common orexigenic hormone which acts as starvation signal by acting on hypothalamic neurons. It acts as adipogenic agent. Increased food intake caused by high levels of ghrelin causes increase fat mass. We investigated the possible beneficial role of ghrelin antagonist on food and water intake in obese and type 2 diabetic mice. Methods: This study was carried out at Department of Physiology, Army Medical College, Rawalpindi in collaboration with National Institute of Health, Islamabad, from Jan to Jun 2013. A total of 50 healthy male BALB/c mice were divided into 5 groups. Group I served as control, groups II and III were obese, and group IV and V were diabetic. Group II, III, IV and V were fed high-fat diet for 4 weeks. Group IV and V were given intraperitoneal (IP) injection of streptozotocin to induce type 2 diabetes mellitus. Ghrelin antagonist was injected IP to Group III and V for 6 days. Food and water intake were measured daily. Body weights were measured twice a week and at the end of experiment. Terminal intracardiac blood extraction was done and samples were analyzed for fasting plasma glucose levels. Results: Food intake decreased significantly in group III (obese with Ghrelin antagonist) and group V (diabetic with Ghrelin antagonist) as compared to controls after injection of ghrelin antagonist. Conclusion: Ghrelin antagonist decreases the food and water intake significantly in obese and type 2 diabetic mice.

Pak J Physiol 2017;13(1):39–42

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Published

31-03-2017

How to Cite

1.
Siddique L, Imran M. EFFECT OF GHRELIN ANTAGONIST ON FOOD AND WATER INTAKE IN OBESE AND TYPE 2 DIABETIC MICE. Pak J Phsyiol [Internet]. 2017 Mar. 31 [cited 2024 Apr. 26];13(1):39-42. Available from: https://pjp.pps.org.pk/index.php/PJP/article/view/265

Issue

Vol. 13 No. 1 (2017): Pakistan Journal of Physiology

Section

Original Article

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Pakistan Journal of Physiology, Pak J Physiol, PJP is FREE for research and academic purposes. It can be freely downloaded and stored, printed, presented, cited and quoted with full reference of, and acknowledgement to the PJP.