PREVENTION AND DISSOLUTION EFFECTS OF SANG SARMAHI ON CALCIUM OXALATE CRYSTALS INDUCED BY GLYOXALATE TREATMENT IN RATS
Background: Development of cost-effective therapy for safe removal of kidney stones is very much desired. The purpose of this study was to evaluate litholytic and anti-urolithic effects of Sang sarmahi on calcium oxalate crystals induced by glyoxalate treatment in rats. Methods: Thirty male Wistar rats were randomly divided into five equal groups; A, B1, B2, C1 and C2. Group A rats were untreated and served as negative controls. The rats in groups B1 and B2 after induction of calcium oxalate crystals were respectively given placebo and Sang sarmahi treatment and served as positive control and curative groups. The rats in groups C1 and C2 were simultaneously administered glyoxylate+placebo, and glyoxylate+Sang sarmahi treatments and respectively served as positive controls and preventive group. Serum samples of rats in groups A, C1 and C2 were measured for the levels of superoxide dismutase, glutathione peroxidase, catalase, calcium, magnesium, oxalate and creatinine. Similarly, left kidney tissue homogenates of rats in groups A, B1 and B2 were analyzed for the levels of reduced glutathione, malondialdehyde, superoxide dismutase, glutathione peroxidase and catalase. Histological slides of right kidney sections of all 30 rats were prepared for microscopic examination. Results: Histological examination of renal tissue sections showed that Sang sarmahi failed to prevent crystallization in kidneys of groups B and C rats. No significant differences were seen in serum and tissue homogenate parameters between positive controls and Sang sarmahi treated rats of both prevention and curative groups. Conclusion: Sang sarmahi failed to exhibit any significant litholytic and anti-urolithic activity.
2. Vijaya T, Kumar S, Ramaro NV, Banu N, Ramarao N. Urolithiasis and its causes â€•Short Review. J Phytopharmcol 2013;2(3):1â€“6.
3. Al-Atar U. Materials characterization of calcium oxalate monohydrate kidney stones. Thesis. Department of Chemistry, Simon Fraser University; Canada: 2009.
4. Wesson JA, Ward MD. Pathological biomineralization of kidney stone elements 2007;3(6):415â€“21.
5. Lopez M, Hoppe B. History, epidemiology and regional diversities of urolithiasis. Pediatr. Nephrol 2010;25(1):49â€“59.
6. Ramello A, Vitale C, Marangella M. Epidemiology of nephrolithiasis. J Nephrol 2000;13(3):S45â€“50.
7. Medina-Escobedo M, Zaidi M, Real-deLeon E, Orosco-Rivadeneyra S. Urolithiasis prevalence and risk factors in Yucatan, Mexico. Salud Publica Mex 2002;44:541â€“5.
8. Abu Abdullah Ibn Al-Qayyim Al-Jauzia. Al Tib-E-Nabvi PBUH Darussalam Publishers; 1970. Chapter 7, page 55â€“7.
9. Chen YH, Liu HY, Chen FJ, Tsai. Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis. Kidney International 2011;80(4):369â€“77.
10. Khan SR. Animal models of kidney stone formation: an analysis. World J Urol 1997;15(4):236â€“43.
11. Oh SY, Kwon JK, Lee SY, Ha MS. A comparative study of experimental rat models of renal calcium oxalate stone formation. J Endourol 2011;25(6):1057â€“61.
12. Fish E, Brown MJ, Danneman PJ, Karas AZ, (Eds). Anesthesia and Analgesia in Laboratory Animals (2nd ed.) San Diego: American College of Laboratory Animal Medicine Series, Academic Press;2008.
13. Mahmoud RH, Ewadh MJ. Clinical assessment of glutathione peroxidase and catalase to the status of malondialdehyde in urolithiasis. Pak J Med Sci 2009;25(5):738â€“43.
14. Sadati ZA, Moslemi D, Parsian H, Vessal M, Mosapour A, Kelagari S. The status of antioxidants, malondialdehyde and some trace elements in serum of patients with breast cancer. Caspian J Intern Med 2016;7(1):31â€“6.
15. Soundararajan P, Mahesh R, Ramesh T, Begum VH. Effect of Aerva lanata on calcium oxalate urolithiasis in rats. Indian J Exp Bio 2006;44(12):981â€“6.
16. Almaghrabi OA. Molecular and biochemical investigations on the effect of quercetin on oxidative stress induced by cisplatin in rat kidney. Saudi J Biol Sci 2015;22(2):227â€“31.
17. Balakrishnan A, Kokilavani R, Gurusamy K, Teepa KS, Sathya M. Effect of ethanolic fruit extract of Cucumis trigonus Roxb on antioxidants and lipid peroxidation in urolithiasis induced wistar albino rats. Anc Sci Life 2011;31(1):10â€“6.
18. Greer CE, Peterson SL, Kiviat NB, Manos MM. PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time. Am J Clin Pathol 1991;95(2):117â€“24.
19. Latendresse JR, Warbrittion AR, Jonassen H, Creasy DM. Fixation of testes and eyes using a modified Davidsonâ€™s fluid: comparison with Bouinâ€™s fluid and conventional Davidsonâ€™s fluid. Toxicol Pathol 2002;30(4):524â€“33.
20. Strobel S, Miller HR, Ferguson A. Human intestinal mucosal mast cells evaluation of fixation and staining techniques. J Clin Pathol 1981;34(8):851â€“8.
21. Gressner OA, Weiskirchen R, Gressner AM. Biomarkers of liver fibrosis clinical translation of molecular pathogenesis or based on liver-dependent malfunction tests. Clinica Chimica Acta 2007;381(2):107â€“13.
22. Rushton HG, Spector M. Effects of magnesium deficiency on intratubular calcium oxalate formation and crystalluria in hyperoxaluric rats. J Urol 1982;127:598â€“604.
23. Brzica H, Breljak D, Burckhardt BC, Burckhardt G, Sabolic. Oxalate: from the environment to kidney stones. Arh Hig Rada Toksikol 2013;64:609â€“30.
24. Thamilselvan V, Menon M, Thamilselvan S. Oxalate at physiological urine concentrations induces oxidative injury in renal epithelial cells: Effect of Î±-tocopherol and ascorbic acid. BJU International 2014;114(1):140â€“50.
25. Meimariduo E, Lobos E, Hothersall JS. Renal oxidative vulnerability due to changes in mitochondrial-glutathione and energy homeostasis in a rat model of calcium oxalate urolithiasis. Am J Physiol Renal Physiol 2006;291:F731â€“F740.
26. Carrasco-Valiente J, Anglada-Curado FJ, Aguilar-Me-lero P, GonzÃ¡lez-Ojeda R, Muntane-Relat J, Padillo-Ruiz FJ, et al. State of acute phase markers and oxidative stress in patients with kidney stones in the urinary tract. Actas Urol Esp 2012;36:296â€“301.
27. Khan SR. Is oxidative stress, a link between nephro-lithiasis and obesity, hypertension, diabetes, chronic kidney disease, metabolic syndrome? Urol Res 2012;40:95â€“112.
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