PROTECTIVE ROLE OF TURMERIC ON BIOCHEMICAL PARAMETERS OF LIVER IN NIMESULIDE INDUCED HEPATIC INJURY IN RATS
Background: Damage to hepatocytes, e.g., necrosis, inflammation and degenerative disease causes release of liver enzymes into the circulation and they are measured both for diagnosis and prognosis of disease. The objective of this study was to see the protective effect of turmeric powder on liver enzymes against Nimesulide-induced hepatic injury in rat model. Methods: Forty (40) male albino Wistar rats were divided into four groups. In group A, normal diet was given for 9 days while in group B, Nimesulide was given for 9 days followed by turmeric powder for next 9 days; while turmeric powder for 9 days followed by Nimesulide for next 9 days was used in group C. Group D received Nimesulide in adjunct with Turmeric powder for 9 days. After 24 hours of feeding, Blood samples from groups A and D were taken for biochemical analysis of serum levels of ALT, AST, Alkaline Phosphatase and Î³-GT at days 0 and 9. For groups B and C blood samples were collected at day 1, day 9 and day 18. The results were statistically analyzed by using SPSS-21 and Statistix 8.1. Results: In control group A, the results were found almost same on day 1 and 9. The group B shows a high value on day 9 in comparison to day 1, but after treatment with turmeric there is a marked reduction towards normal in all parameters on day 18. The group C show initially reduction in the values of all parameter on day 9 after taking the turmeric powder but there is an incline after taking Nimesulide. The group D received Nimesulide in adjunct with Turmeric powder for 9 days show slight increase in the results from day 1 in all parameters. The results were found significant (p<0.05) in the three treated groups. Conclusion: Turmeric has hepatoprotective properties against hepatotoxicity produced by Nimesulide.
Keywords: Turmeric, Hepatic injury, LFTs, Nimesulide, Enzymes, Hepatotoxicity
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2. Jakobek L. Interactions of polyphenols with carbohydrates, lipids and proteins. Food Chem 2015;175:556â€“67.
3. Gupta SC, Kismali G, Aggarwal BB. Curcumin, a component of turmeric: from farm to pharmacy. Biofactors 2013;39(1):2â€“13.
4. Nasri H, Sahinfard N, Rafieian M, Rafieian S, Shirzad M, Rafieian-Kopaei M. Turmeric: A spice with multifunctional medicinal properties. J Herbmed Pharmacol 2014;3(1):5â€“8.
5. Saraswati TR, Manalu W, Ekastuti DR, Kusumorini N. Increased egg production of Japanese quail (Cortunix japonica) by improving liver function through turmeric powder supplementation. Int J Poult Sci 2013;12:601â€“4.
6. Casas-Grajales S, Muriel P. Antioxidants in liver health. World J Gastrointest Pharmacol Ther 2015;6(3):59â€“72.
7. Sreejayan N, Rao MN. Free radical scavenging activity of curcuminoids. Arzneimittelforschung 1996,46(2):169â€“71.
8. Sreejayan N, Rao MN. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49(1):105â€“7.
9. Pozharitskaya ON, Ivanova. SA, Shikov AN, Makarov VG. Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTCL-DPPH method. Phytochem Anal 2008;19(3):236â€“43.
10. Osawa T, Sugiyama Y, Inayoshi M, Kawakishi S. Antioxidative activity of tetrahydrocurcuminoids. Biosci Biotechnol Biochem 1995;59:1609â€“12.
11. Song EK, Cho H, Kim JS, Kim NY, An NH, Kim JA, et al. Diarylheptanoids with free radical scavenging and hepatoprotective activity in vitro from Curcuma longa. Planta Med 2001;67:876â€“7.
12. National Research Council. Guidance for the Description of Animal Research in Scientific Publications. Washington DC: National Academic Press; 2011. https://doi.org/10.17226/13241
13. GarcÃa-Nino WR, Pedraza-ChaverrÃ J. Protective effect of curcumin against heavy metals-induced liver damage. Food Chem Toxicol 2014;69:182â€“201.
14. Palipoch S, Punsawad C, Koomhin P, Suwannalert P. Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress. BMC Complement Altern Med 2014;14:111.
15. Nguyen V, Huang J, Doan V, Lin X, Tang X, Huang Y, et al. Hepatoprotective effects of Yulangsan polysaccharide against nimesulide-induced liver injury in mice. J Ethnopharmacol 2015;172:273â€“80.
16. Donati M, Conforti A, Lenti MC, Capuano A, Bortolami O, Motola D, et al. Risk of acute and serious liver injury associated to nimesulide and other NSAIDs: data from drugâ€induced liver injury case-control study in Italy. Br J Clin Pharmacol 2016;82(1):238â€“48.
17. Bernardes SS, Souza-Nogueira A, Moreira EG, Kishima MO, Guembarovski AF, Turini TL, et al. Nimesulide-induced fatal acute liver failure in an elderly woman with metastatic biliary adenocarcinoma. A case report. Sao Paulo Med J 2015;133(4):371â€“6.
18. Kim Y, You Y, Yoon HG, Lee YH, Kim K, Lee J, et al. Hepatoprotective effects of fermented Curcuma longa L. on carbon tetrachloride-induced oxidative stress in rats. Food Chemistry 2014;151:148â€“53.
19. Singh I, Vetriselvan S, Shankar J, Gayathiri S, Hemah C, Shereenjeet G, et al. Hepatoprotective activity of aqueous extract of Curcuma longa in ethanol induced hepatotoxicity in albino Wistar rats. Int J Phytopharmacol 2012;3(3):226â€“33.
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