NIGELLA SATIVA: ROLE IN IRON OVERLOAD

  • Maryam Naeem
  • Muniba Razaq
  • Uzma Naeem IIMC - Riphah university
  • Saima Rafique
  • Khalida Ajmal
  • Tasneem Murad
Keywords: Thalassemia, Iron overload, Nigella stiva, Mice, Clinical trial

Abstract

Background: In thalassemia patients, iron is overloaded because of multiple blood transfusions. This overloaded iron can damage the organs badly which is a major cause of death in these patients. Iron overload in thalassemia major can only be treated by removing the excessive iron through different chelating agents. Commercially available chelating agents are costly and associated with multiple adverse effects. Effectiveness of naturally present chelators is under study. This study was conducted to explore the chelating effect of one of commonly used herb Nigella sativa. Methods: This experimental, randomized controlled trial was conducted in Zoology Department, Government College University, Lahore in its multidisciplinary laboratory. A total of 36 male albino mice were divided into three groups, 12 in each group. Iron was overloaded in groups 2 and 3 by intravenous injections of iron dextran (0.1 ml/g body weight) for 15 days on daily basis. After 15 days, iron dextran injection was discontinued, and mice were allowed to feed on Nigella sativa (200 mg/Kg body weight) for further 15 days. Blood sampling was done at baseline, 15, and 30 days to analyse iron concentration in serum, heart, kidney, and liver. Data was analysed using Student’s t-test. Results: The artificially administered iron in mice increased the iron levels in serum, liver, heart and kidneys to a significant level while administration of N. sativa significantly decreased these levels. Conclusione propose clinical trials of Nigella sativa in thalassemia major patients as an adjunct therapy to explore its efficacy and safety.

Pak J Physiol 2021;17(2):20–3

References

Sharma DC, Arya A, Kishor P, Woike P, Bindal J. Overview on thalasemias: A review article. Med Res Chronicles 2017;4(3):325–37.

Jones G, Goswami SK, Kang H, Choi HS, Kim J. Combating iron overload: a case for deferoxamine-based nanochelators. Nanomedicine (Lond) 2020;15(13):1341–56.

Piperno A, Pelucchi S, Mariani R. Inherited iron overload disorders. Transl Gastroenterol Hepatol 2020;5:25.

Kontoghiorghe CN, Kontoghiorghes GJ. Efficacy and safety of iron-chelation therapy with deferoxamine, deferiprone, and deferasirox for the treatment of iron-loaded patients with nontransfusion-dependent thalassemia syndromes. Drug Des Devel Ther 2016;10:465–81.

Hoffbrand AV, Cohen A, Hershko C. Role of deferiprone in chelation therapy for transfusional iron overload. Blood 2003;102(1):17–24.

Kishwar F, Mahmood I, Mahmood T, Qamar-ul-Haq. Thymol, an active constituent of nigella sativa, could reduce toxicity of some trace metals Fe(III),Cr(VI),Cu(II),V(IV) and Co(II). Eur Res J 2015;1(6):1110–25.

Jacobs A, Miller F, Worwood M, Beamish MR, Wardrop CA. Ferritin in the serum of normal subjects and patients with iron deficiency and iron overload. BMJ 1972;4(5834):206–8.

Das A, Chaudhuri D, Ghate NB, Panja S, Chatterjee A, Mandal N. Protective effect of Clerodendrum colebrookianum leaves against iron-induced oxidative stress and hepatotoxicity in Swiss albino mice. Indian J Exp Biol 2015;53(5):281–91.

Mirzaei A, Delaviz H, Mirzaei M, Tolooei M. The effects of Medicago sativa and Allium porrum on iron overload in rats. Glob J health Sci 2015;7 (7 Spec No):137–42.

Danladi J, Ahmed SA, Akpulu SP, Owolagba GK, Iduh MU, Mairiga AA. Protective effect of cool extraction of black seed (Nigella Sativa) oil against CCl4-induced oxidative damages in wistar rats testis. IOSR-J Pharm Bio Sci 2013;5(2):68–74.

Olabinri, BM, Eniyansoro OO, Okoronkwo CO, Olabinri PF, Olaleye MT. Evaluation of chelating ability of aqueous extract of Tetracarpidium conophorum (African walnut) in vitro. Int J App Res Nat Prod 2010;3(3):13–8.

Wongjaikam S, Kumfu S, Khamseekaew J, Sripetchwandee J, Srichairatanakool S, Fucharoen S, Chattipakorn SC, et al. Combined iron chelator and antioxidant exerted greater efficacy on cardioprotection than monotherapy in iron-overloaded rats. PLoS ONE 2016;11(7):0159414.

Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M. Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine 2002;9(1):69–74.

Karlsson M, Ekstedt M, Dahlström N, Forsgren MF, Ignatova S, Norén B, et al. Liver R2* is affected by both iron and fat: A dual biopsy‐validated study of chronic liver disease. J Magn Reson Imaging 2019;50(1):325–33.

Downloads

Download data is not yet available.
Published
2021-06-30
How to Cite
1.
Naeem M, Razaq M, Naeem U, Rafique S, Ajmal K, Murad T. NIGELLA SATIVA: ROLE IN IRON OVERLOAD. PJP [Internet]. 30Jun.2021 [cited 27Sep.2021];17(2):20-3. Available from: http://pjp.pps.org.pk/index.php/PJP/article/view/1303