Restoration of renal glutathione levels and histoarchitecture by N-acetylcysteine post cyclophosphamide exposure in rats

  • Maryam Saqib Army Medical College
  • Bushra Tayyaba Khan Army Medical College
  • Mahwish Gul Ayub Medical College
  • Sumetha Yaseen Army Medical College
  • Syeda Urooj Zaidi Army Medical College
  • Mahjabeen Shareef Army Medical College
Keywords: Cyclophosphamide, Glutathione, Histoarchitecture, N-acetylcysteine, Nephrotoxicity

Abstract

Objective: To evaluate the protective potential of N-acetylcysteine against cyclophosphamide induced nephrotoxicity in rats.

Study design: Laboratory-based randomized controlled trial.

Place and Duration of Study: Department of Pharmacology and Therapeutics in collaboration with Clinicopathologic Laboratory, Army Medical College, Rawalpindi and National Institute of Health, Islamabad from  March to July 2018.

Material and Methods: A total of eighteen rats were divided into three equal groups. Group I served as the untreated control, Group II received a single intraperitoneal injection of cyclophosphamide (200mg/kg) and the Group III was administered an intraperitoneal injection of N-acetylcysteine (100mg/kg), once daily for five consecutive days followed by a single injection of cyclophosphamide one hour after the last dose. Twenty four hours after cyclophosphamide administration, the animals were sacrificed and the kidneys were evaluated for glutathione levels and histopathological changes. The results were statistically analyzed on SPSS version 21, for quantitative analysis one way ANOVA followed by post hoc Tukey test and for histopathologic analysis, Chi-Square test was applied.

Results: In the renal tissue, cyclophosphamide depleted the glutathione levels from the normal mean of 1.17ng/ml to 0.34ng/ml with p ≤ 0.05, alongside notable histopathologic changes including multiple foci of hemorrhages and dense leukocytic infiltrates. N-acetylcysteine reestablished the glutathione pools by raising the mean value upto 0.93ng/ml and preserved the normal histoarchitecture.

Conclusion: N-acetylcysteine can attenuate cyclophosphamide induced nephrotoxicity by preserving the normal glutathione levels and histoarchitecture.

Keywords: Cyclophosphamide, Glutathione, Histoarchitecture, N-acetylcysteine, Nephrotoxicity

Author Biographies

Bushra Tayyaba Khan, Army Medical College

Associate Professor, Department of Pharmacology and Therapeutics, Army Medical College

Mahwish Gul, Ayub Medical College

Lecturer, Department of Pharmacology and Therapeutics, Ayub Medical College

Mahjabeen Shareef, Army Medical College

Associate Professor, Department of pharmacology and therapeutics, Army Medical College

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Published
2019-06-30
How to Cite
1.
Saqib M, Khan B, Gul M, Yaseen S, Zaidi S, Shareef M. Restoration of renal glutathione levels and histoarchitecture by N-acetylcysteine post cyclophosphamide exposure in rats. PJP [Internet]. 30Jun.2019 [cited 9Dec.2019];15(2):33-7. Available from: http://pjp.pps.org.pk/index.php/PJP/article/view/1098